# US 2021/0277352 A1 · Enrichment supplement for antimicrobal matrices

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2021/0277352 A1
- Document ID: US20210277352A1
- Status: Published application
- Kind: A1
- Application number: 17/252,401
- Filed: 2019-06-13
- Published: 2021-09-09
- Earliest claimed: 2018-06-20
- Assignees: 3M Innovative Properties Company
- Applicants: 3M Innovative Properties Company
- Inventors: Gregory W. Sitton; Wilfredo Dominguez-Nunez
- Web page: https://patentgrep.com/patent/US20210277352A1

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- C12Q 1/045: {Culture media therefor}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Salts of Fe (II), Zn (II), or both used in methods for enriching food matrices containing antimicrobial compounds. A kit comprising salts of Fe (II), Zn (II) useful for such methods.

## Claims

### Claim 1

A method of growing of a target microorganism comprising incubating a matrix having one or more antimicrobial compounds that are active against the target microorganism in the presence of an Fe(II) salt or a Zn(II) salt.

### Claim 2 (depends on 1)

The method of claim 1, wherein the matrix is a food matrix.

### Claim 3 (depends on 1)

The method of claim 1, wherein the matrix comprises a nut matrix, a food matrix, or a combination thereof.

### Claim 4 (depends on 1)

The method of claim 1, wherein the target microorganism is Listeria or Salmonella.

### Claim 5 (depends on 1)

The method of claim 1, wherein the microorganism is neither a yeast nor an iron oxidizing or reducing microorganism.

### Claim 6 (depends on 1)

The method of claim 1, further comprising dissolving or dispersing the matrix in a culture broth containing an enrichment medium.

### Claim 7 (depends on 6)

The method of any of the claim 6, further comprising admixing the matrix, the culture broth, and the Fe (II) or Zn (II) salt, wherein the Fe(II) salt or Zn(II) salt is at a concentration of 100 micromolar or greater.

### Claim 8 (depends on 6)

The method of any of the claim 6, further comprising admixing the matrix, the culture broth, and the Fe (II) or Zn (II) salt, wherein the Fe(II) salt or Zn(II) salt is at a concentration of 1,000 micromolar or less.

### Claim 9 (depends on 7)

The method of claim 7, wherein the enrichment medium is a selective enrichment medium for the target microorganism.

### Claim 10 (depends on 1)

The method of claim 1, wherein the Zn (II) salt is in the form of zinc chloride.

### Claim 11 (depends on 1)

The method of claim 1, wherein the Fe (II) salt is in the form of ferrous sulfate.

### Claim 12 (depends on 1)

The method of claim 1, further comprising detecting the presence of a target microorganism.

### Claim 13

A kit comprising:
enrichment media that is selective for Salmonella or Listeria; and
a Zn (II) salt or an Fe (II) salt.

### Claim 14 (depends on 13)

The kit of claim 13, wherein the Zn (II) salt or an Fe (II) salt is a component of the enrichment media.

### Claim 15 (depends on 13)

The kit of claim 13, wherein the Zn (II) salt or Fe (II) salt is separate from the enrichment media.

### Claim 16 (depends on 8)

The method of claim 8, wherein the enrichment medium is a selective enrichment medium for the target microorganism.

### Claim 17 (depends on 4)

The method of claim 4, wherein the enrichment medium is a selective enrichment medium for the target microorganism.