# US 2021/0277350 A1 · Particle separation and analysis

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- Number: US 2021/0277350 A1
- Document ID: US20210277350A1
- Status: Published application
- Kind: A1
- Application number: 16/605,985
- Filed: 2018-03-27
- Published: 2021-09-09
- Earliest claimed: 2018-03-27
- Assignees: Hewlett-Packard Development Company, L.P.
- Applicants: Hewlett-Packard Development Company, L.P.
- Inventors: Viktor Shkolnikov; Alexander Govyadinov
- Web page: https://patentgrep.com/patent/US20210277350A1

## CPC

- C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)}
- C12M 47/06: {Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003)}
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses
- C12Q 1/6844: Nucleic acid amplification reactions
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

An example system includes an input channel to receive particles through a first end, a separation chamber, at least two output channels, an integrated pump to facilitate flow through the separation chamber and a cell analysis portion. The separation chamber is in fluid communication with a second end of the input channel. The separation chamber has a passive separation structure including an array of columns spaced apart to facilitate separation of particles into at least two flow paths based on a size of the particles. The size associated with a first flow path of the at least two flow paths corresponds to a cell. A first output channel is to receive the first flow path corresponding to a cell. The cell analysis portion is coupled to the first output channel and is to perform at least one analysis associated with cells in the first output channel.

## Claims

### Claim 1

A system, comprising:
an input channel having a first end and a second end to receive particles through the first end;
a separation chamber in fluid communication with the second end of the input channel, the separation chamber having a passive separation structure, the passive separation structure including an array of columns spaced apart to facilitate separation of particles in a flow into at least two flow paths based on a size of the particles, wherein the size associated with a first flow path of the at least two flow paths corresponds to a cell;
at least two output channels, each output channel coupled to the separation chamber to receive separated particles, wherein a first output channel is to receive the first flow path corresponding to a cell;
an integrated pump to facilitate flow through the separation chamber, the integrated pump being positioned within at least one of the input channel or one of the at least two output channels; and
a cell analysis portion coupled to the first output channel, the cell analysis portion being to perform at least one analysis associated with cells in the first output channel.

### Claim 2 (depends on 1)

The system of claim 1, wherein the array of columns is arranged to deterministically direct a particle in a flow colliding with the columns to a first side if the particle in the flow is smaller than a critical diameter and to a second side if the particle in the flow is larger than the critical diameter.

### Claim 3 (depends on 1)

The system of claim 1, wherein the integrated pump is an inertial pump.

### Claim 4 (depends on 1)

The system of claim 1, wherein the integrated pump is a drop ejector.

### Claim 5 (depends on 1)

The system of claim 1, wherein the integrated pump includes a thermal inkjet resistor or a piezo element.

### Claim 6 (depends on 1)

The system of claim 1, further comprising a cell lysis portion to lyse cells in the first output channel.

### Claim 7 (depends on 6)

The system of claim 6, further comprising:
a reagent input channel in fluid communication with the first output channel, wherein flow from the reagent input channel is to mix with flow of separated particles from the separation chamber in the first output channel, the flow from the reagent input channel including a lysing agent.

### Claim 8 (depends on 6)

The system of claim 6, further comprising:
a detection portion to detect hybridization of biological material from the lysed cells.

### Claim 9 (depends on 8)

The system of claim 8, further comprising:
a biological material amplification portion to amplify biological material from the lysed cells, the biological material amplification portion being positioned to receive biological material from the cell lysis portion and to provide the amplified biological material to the detection portion.

### Claim 10 (depends on 6)

The system of claim 6, further comprising:
a biological material amplification portion to amplify biological material from the lysed cells;
a sequencing portion to obtain a genetic sequence from the amplified biological material; and
a mapping portion to identify a condition based on the genetic sequence.

### Claim 11 (depends on 10)

The system of claim 10, wherein the mapping portion includes a machine learning portion to facilitate identification of condition using pattern matching.

### Claim 12 (depends on 1)

The system of claim 1, wherein the cell analysis portion includes an antibody matching portion, the antibody matching portion including an array of cell identification regions, each cell identification region including antibodies to bind to a corresponding cell.

### Claim 13

A system, comprising:
an input channel to flow particles therethrough;
a cell separation chamber in fluid communication with the input channel, the cell separation chamber including an array of columns spaced apart to facilitate separation of particles in a flow into at least two flow paths based on a size of the particles, wherein the size associated with a first flow path of the at least two flow paths corresponds to cell-sized particles;
at least two output channels, each output channel coupled to the separation chamber to receive separated particles, wherein a first output channel is to receive the first flow path corresponding to the cell-sized particles;
a cell lysing portion to mix a lysing agent with the cell-sized particles, the lysing agent to release biological material from cells;
an integrated pump to facilitate flow through the separation chamber; and
a cell analysis portion to receive biological material from lysed cells, the cell analysis portion including at least one detection portion to identify a characteristic of the biological material.

### Claim 14 (depends on 1)

The system of claim 1, wherein the cell lysing portion includes a reagent input channel in fluid communication with the first output channel.

### Claim 15

A system, comprising:
an input channel to flow particles therethrough;
a cell separation chamber in fluid communication with the input channel, the cell separation chamber including an array of columns spaced apart to facilitate separation of particles in a flow into at least two flow paths based on a size of the particles, wherein the size associated with a first flow path of the at least two flow paths corresponds to cell-sized particles;
at least two output channels, each output channel coupled to the separation chamber to receive separated particles, wherein a first output channel is to receive the first flow path corresponding to the cell-sized particles;
a cell lysing portion to mix a lysing agent with the cell-sized particles, the lysing agent to release biological material from cells;
an integrated pump to facilitate flow through the separation chamber; and
a biological material amplification portion to amplify biological material from lysed cells.