Crp capture/detection of gram positive bacteria
AssigneePresident and Fellows of Harvard College
InventorsAlexander L. WATTERS, Donald E. INGBER, Mark J. CARTWRIGHT, Michael SUPER, Martin ROTTMAN, Evangelia MURRAY, Brendon DUSEL
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Abstract
Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.
Claims16
A method for determining the presence or absence of a microbe in a sample, comprising:
- (i) contacting a test sample with the microbe-targeting molecule;
- and
- (ii) detecting binding of a microbe to the microbe-targeting molecule,
- wherein the microbe is present in the test sample if binding is detected and wherein the microbe-targeting molecule comprises:
- a. at least one first domain comprising at least a portion of a c-reactive protein (CRP);
- b. at least one second domain comprising at least a portion of a domain selected from the group consisting of:
- (i) Fc region of an immunoglobulin;
- (ii) microbe-binding domain of a microbe-binding protein, wherein the microbe-binding protein is not CRP;
- (iii) neck region of a lectin;
- (iv) a detectable label;
- (v) domain for conjugation to surface of a carrier scaffold;
- (vi) pattern recognition receptor domain of CRP;
- and
- (vii) any combinations of (i)-(vi);
- and
- c. a linker conjugating the first and second domains, and
- wherein the microbe-targeting molecule is a multimeric molecule.
Dependencies are inferred from the claim text.