# US 2021/0276904 A1 · Novel microorganisms for decomposing oil and fat containing fatty acid

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- Number: US 2021/0276904 A1
- Document ID: US20210276904A1
- Status: Published application
- Kind: A1
- Application number: 17/258,132
- Filed: 2019-07-05
- Published: 2021-09-09
- Earliest claimed: 2018-07-06
- Assignees: National University Corporation Tokai National Higher Education and Research System
- Applicants: National University Corporation Tokai National Higher Education and Research System
- Inventors: Katsutoshi Hori
- Web page: https://patentgrep.com/patent/US20210276904A1

## CPC

- C02F 3/343: {for digestion of grease, fat, oil}
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- C02F 2103/32: from the food or foodstuff industry, e.g. brewery waste waters
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present disclosure provides a new microorganism with an ability to decompose oil and fat. In one embodiment, the microorganism is from the family Burkholderiaceae. In one embodiment, the microorganism is from the genus Burkholderia. In one embodiment, the microorganism is Burkholderia arboris, or Burkholderia cepacia complex, or the like. In one embodiment, the microorganism of the present disclosure has an ability to assimilate trans fatty acid. In one embodiment, the microorganism of the present disclosure has an ability to decompose trans fatty acid. In one embodiment, the microorganism of the present disclosure has an ability to decompose trans fatty acid-containing oil and fat. In one embodiment, the microorganism of the present disclosure has an ability to assimilate trans fatty acid-containing oil and fat.

## Claims

### Claim 1

A bacterium in family Burkholderiaceae, having an ability to assimilate or decompose trans fatty acid-containing oil and fat or trans fatty acid.

### Claim 2 (depends on 1)

The bacterium in the family Burkholderiaceae of claim 1, having an ability to assimilate trans fatty acid.

### Claim 3 (depends on 1)

The bacterium in the family Burkholderiaceae of claim 1, having an ability to decompose trans fatty acid.

### Claim 4 (depends on 1)

The bacterium in the family Burkholderiaceae of claim 1, having an ability to decompose trans fatty acid-containing oil and fat.

### Claim 5

A bacterium in family Burkholderiaceae, having an ability to decompose oil and fat at 15° C.

### Claim 6 (depends on 1)

The bacterium in the family Burkholderiaceae of claim 1, wherein the ability to assimilate or decompose is retained at 15° C.

### Claim 7 (depends on 1)

The bacterium of claim 1, which is a bacterium of genus Burkholderia.

### Claim 8 (depends on 1)

The bacterium of claim 1, which is a microorganism belonging to Burkholderia arboris, Burkholderia ambifaria, or Burkholderia cepacia complex.

### Claim 9 (depends on 1)

The bacterium of claim 1, which is a KH-1 strain (bacterial strain identified by accession number NITE BP-02731), a KH-1AL1 strain (bacterial strain identified by receipt number NITE ABP-02977), a KH-1AL2 strain (bacterial strain identified by receipt number NITE ABP-02978), or a KH-1AL3 strain (bacterial strain identified by receipt number NITE ABP-02979) of a bacterium in the genus Burkholderia, or a derivative strain thereof that has a feature of the bacterium of claim 1.

### Claim 10 (depends on 1)

An oil decomposing agent comprising the bacterium of claim 1.

### Claim 11 (depends on 10)

The oil decomposing agent of claim 10, further comprising an oil treating component.

### Claim 12 (depends on 1)

A kit for decomposing oil, comprising the bacterium of claim 1 or an oil decomposing agent comprising the bacterium; and an additional oil treating component.

### Claim 13 (depends on 1)

A method of decomposing and removing oil, comprising causing the bacterium of claim 1 or an oil decomposing agent comprising the bacterium to act on a subject of treatment.

### Claim 14 (depends on 13)

The method of claim 13, wherein the subject of treatment comprises trans fatty acid or trans fatty acid-containing oil and fat.

### Claim 15 (depends on 1)

The bacterium in the family Burkholderiaceae of claim 1, having an ability to assimilate trans fatty acid-containing oil and fat.