# US 2021/0276060 A1 · Soil remediation through beneficial microbes > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0276060 A1 - Document ID: US20210276060A1 - Status: Published application - Kind: A1 - Application number: 17/195,214 - Filed: 2021-03-08 - Published: 2021-09-09 - Earliest claimed: 2020-03-06 - Assignees: New Life Biosciences, LLC - Applicants: New Life Biosciences LLC - Inventors: Marc Rodriguez - Web page: https://patentgrep.com/patent/US20210276060A1 ## CPC - B09C 1/10: microbiologically, {biologically} or by using enzymes - A61K 35/37: Digestive system - B09C 1/002: {involving in-situ ground water treatment} - B09C 1/105: {using fungi or plants} - C02F 3/10: Packings; Fillings; Grids (packing elements in general B01J19/30, B01J19/32) - C02F 3/341: {Consortia of bacteria} - C02F 3/348: {characterised by the way or the form in which the microorganisms are added or dosed} - C12N 1/20: Bacteria; Culture media therefor - B09C 2101/00: In situ - C02F 2101/306: {Pesticides} - C02F 2305/06: Nutrients for stimulating the growth of microorganisms - B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A method and composition are provided for soil remediation. A method is provided for breaking down an organic chemical agent, comprising providing a plurality of sporulated microbes to the chemical agent. A composition is provided for breaking down an organic chemical agent, comprising a plurality of sporulated microbes. ## Claims ### Claim 1 A method for breaking down an organic chemical agent, comprising providing a plurality of sporulated microbes to the chemical agent, the sporulated microbes having a microbe efficacy, and the chemical agent having a chemical agent efficacy. ### Claim 2 (depends on 1) The method as in claim 1, wherein the chemical agent comprises at least one of: an agricultural agent; a petroleum agent; an industrial site agent; and a metabolite of the chemical agent. ### Claim 3 (depends on 1) The method as in claim 1, wherein the chemical agent comprises at least one of a herbicide, an insecticide, and a fungicide. ### Claim 4 (depends on 1) The method as in claim 1, wherein the chemical agent comprises at least one of: glyphosate; dicamba; 2,4-dichlorophenoxyacetic acid; Atrazine; fomesafen; triazine; sulfonylurea; imidacloprid; clothianidin; and thiamethoxam. ### Claim 5 (depends on 1) The method as in claim 1, wherein providing the plurality of sporulated microbes to the chemical agent comprises providing a mixture of the sporulated microbes and the chemical agent. ### Claim 6 (depends on 5) The method as in claim 5, further wherein the mixture retains the microbe efficacy and the chemical agent efficacy. ### Claim 7 (depends on 1) The method as in claim 1, wherein providing the plurality of sporulated microbes comprises distribution on at least one of: a plurality of plants after the plurality of plants has been treated with an application of the organic chemical agent; a soil that has been treated with an application of the organic chemical agent; a soil that contains the organic chemical agent; a sidewalk; a park; a residential lawn; a residential driveway; a sports field; a golf course; and a. a water source. ### Claim 8 (depends on 1) The method as in claim 1, wherein the plurality of sporulated microbes comprises at least one of: a pellet; a powder; a liquid; and granules. ### Claim 9 (depends on 1) The method as in claim 1, wherein providing the plurality of sporulated microbes comprises providing a concentration of sporulated microbes sufficient to break down the chemical agent. ### Claim 10 (depends on 1) The method as in claim 1, wherein providing the plurality of sporulated microbes comprises providing a concentration sufficient to reduce an active form of the chemical agent in a plant food product. ### Claim 11 (depends on 9) The method as in claim 9, wherein providing a concentration comprises providing a concentration that allows planting a crop after a set time without risk of an injury to the crop. ### Claim 12 (depends on 11) The method as in claim 11, wherein the plurality of sporulated microbes comprises a concentration of sporulated microbes based on at least one of: an amount of the chemical agent; a type of chemical agent; and a soil type. ### Claim 13 (depends on 1) The method as in claim 1, wherein the plurality of sporulated microbes comprises a solution with a concentration of sporulated microbes at least 1×1011 CFU/Gallon. ### Claim 14 (depends on 1) The method as in claim 1, wherein the plurality of sporulated microbes comprises a solution with a pH lower than 8.5. ### Claim 15 (depends on 1) The method as in claim 1, wherein the sporulated microbes comprise gram-negative and gram-positive bacteria that metabolize a carbon bond or a covalent bond. ### Claim 16 (depends on 1) The method as in claim 1, wherein the sporulated microbes comprise motile bacteria that metabolize a carbon or a covalent bond. ### Claim 17 (depends on 1) The method as in claim 1, wherein the sporulated microbes comprise Bacillus bacteria that metabolize a carbon or a covalent bond. ### Claim 18 (depends on 17) The method as in claim 17, wherein the sporulated microbes comprise at least two of: a. Bacillus licheniformis; b. Bacillus coagulans; c. Bacillus subtilis; d. Bacillus pumilus; e. Bacillus megaterium; and f. Bacillus amyloliquefaciens. ### Claim 19 (depends on 15) The method as in claim 15, further wherein the sporulated microbes comprise cellulose and lignan composting bacteria, for breaking down at least one of crop waste and weed waste. ### Claim 20 (depends on 1) The method as in claim 1, wherein providing the plurality of sporulated microbes further comprises providing at least one of a nutrient, an extract, a seaweed, or a sugar as a food source for the microbes. ### Claim 21 A composition for breaking down an organic chemical agent, comprising a plurality of sporulated microbes. ### Claim 22 (depends on 21) The composition as in claim 21, further comprising the organic chemical agent. ### Claim 23 (depends on 21) The composition as in claim 21, wherein the sporulated microbes comprise a solution with a density of at least 1×1011 CFU/Gallon. ### Claim 24 (depends on 21) The composition of claim 21, wherein the plurality of sporulated microbes comprises at least two of: a. Bacillus licheniformis; b. Bacillus coagulans; c. Bacillus subtilis; d. Bacillus pumilus; e. Bacillus megaterium; and f. Bacillus amyloliquefaciens. ### Claim 25 (depends on 21) The composition of claim 21, further comprising cellulose and lignan composting bacteria, for breaking down at least one of a crop waste and a weed waste. ### Claim 26 (depends on 21) The composition of claim 21, further comprising at least one of a nutrient, an extract, or a sugar as a food source for the microbes.