# US 2021/0276022 A1 · Bio-sensor having interdigitated micro-electrode using dielectric substance electrophoresis, and method for detecting bio-material using the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0276022 A1 - Document ID: US20210276022A1 - Status: Published application - Kind: A1 - Application number: 17/259,074 - Filed: 2019-07-10 - Published: 2021-09-09 - Earliest claimed: 2018-07-11 - Assignees: Xyz Platform Inc. - Applicants: Xyz Platform Inc. - Inventors: Kyo Seon HWANG; Hye Jin KIM - Web page: https://patentgrep.com/patent/US20210276022A1 ## CPC - B03C 5/005: {Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength} - B03C 5/026: {using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]} - G01N 27/3276: {being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68)} - G01N 27/44717: {Arrangements for investigating the separated zones, e.g. localising zones} - G01N 27/44791: {Microapparatus (sample containers with integrated microfluidic structures B01L3/5027)} - G01N 33/5438: {Electrodes} - B03C 2201/24: for measuring or calculating of parameters, e.g. efficiency - B03C 2201/26: for use in medical or biological applications - B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A biosensor of the present invention comprises: a first microelectrode and a second microelectrode arranged to intersect in a comb shape on a substrate; and a plurality of receptors fixed in a space between the microelectrodes to specifically react with a target biomaterial. In particular, a micropattern of a conductive material is formed in the space between the microelectrodes. Accordingly, greater electric field intensity can be obtained compared to a biosensor without micropatterns, thereby concentration of the target biomaterial using dielectric electrophoretic forces can be performed more efficiently. In addition, damage to biomolecules can be prevented by lowering the intensity of a voltage for a dielectric electrophoresis phenomenon and the biosensor can be easily commercialized as a health care sensor for diagnosing diseases. ## Claims ### Claim 1 A dielectrophoresis-used microelectrode biosensor comprising: a first microelectrode with a plurality of first protruding electrodes arranged in a comb shape on a substrate; a second microelectrode arranged to intersect with each first protruding electrode formed on the first microelectrode and arranged with a plurality of second protruding electrodes arranged in a comb shape; a conductive-materialed micropattern formed in a space between the first microelectrode and the second microelectrode; and a plurality of receptors fixed in a space between the first microelectrode and the second microelectrode to specifically react with a target biomaterial. ### Claim 2 (depends on 1) The dielectrophoresis-used microelectrode biosensor according to claim 1, wherein the first microelectrode and the second microelectrode are applied with an AC (Alternating Current) for generating a dielectrophoresis force. ### Claim 3 (depends on 1) The dielectrophoresis-used microelectrode biosensor according to claim 1, wherein the micropattern is formed in any one shape of square, rectangular and line(ar) shapes. ### Claim 4 (depends on 3) The dielectrophoresis-used microelectrode biosensor according to claim 3, wherein length of each side of the square is greater than 2.0 μm but less than 3.0 μm. ### Claim 5 (depends on 3) The dielectrophoresis-used microelectrode biosensor according to claim 3, wherein length of one side of the square is greater than 2.0 μm but less than 3.0 μm while length of the other side of the square is greater than 7.0 μm but less than 8.0 μm. ### Claim 6 (depends on 3) The dielectrophoresis-used microelectrode biosensor according to claim 3, wherein width of line(ar) micropattern is greater than 2.0 μm but less than 3.0 μm. ### Claim 7 (depends on 1) The dielectrophoresis-used microelectrode biosensor according to claim 1, wherein intensity of AC generating the dielectrophoresis is greater than 0.25V but less than 0.35V, and frequency is 50 MHz. ### Claim 8 (depends on 1) The dielectrophoresis-used microelectrode biosensor according to claim 1, wherein the target biomaterial includes amyloid beta protein, and the receptor includes amyloid beta antibody. ### Claim 9 (depends on 1) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 1; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 10 (depends on 9) The method according to claim 9, wherein the voltage applied between the first microelectrode and the second microelectrode is an AC for generating a dielectrophoresis force, and the magnitude of the voltage is greater than 0.25V but less than 0.35V, and frequency is 50 MHz. ### Claim 11 (depends on 9) The method according to claim 9, wherein weight of the target bio material is greater than 4.0 kDa but less than 5.0 kDa. ### Claim 12 (depends on 9) The method according to claim 9, wherein the target biomaterial includes amyloid beta protein. ### Claim 13 (depends on 2) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 2; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 14 (depends on 3) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 3; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 15 (depends on 4) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 4; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 16 (depends on 5) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 5; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 17 (depends on 6) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 6; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 18 (depends on 7) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 7; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance. ### Claim 19 (depends on 8) A method for detecting bio material using a biosensor, comprising: applying a voltage to the first microelectrode and the second microelectrode using the dielectrophoresis according to claim 8; measuring an impedance between the first microelectrode and the second microelectrode; and calculating one or more of presence or absence of target biomaterial and concentration thereof based on the measured impedance.