# US 2021/0276009 A1 · Micro chamber plate

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- Number: US 2021/0276009 A1
- Document ID: US20210276009A1
- Status: Published application
- Kind: A1
- Application number: 17/330,009
- Filed: 2021-05-25
- Published: 2021-09-09
- Earliest claimed: 2013-11-20
- Assignees: Bioneer Corporation
- Applicants: Bioneer Corporation
- Inventors: Gu-Young SONG; Han Oh Park; Won-Seok Jang; Jung A. Bae
- Web page: https://patentgrep.com/patent/US20210276009A1

## CPC

- B01L 3/50853: {with covers or lids (closures for test tubes B01L3/50825)}
- C12Q 1/686: Polymerase chain reaction [PCR]
- B01L 2200/0689: Sealing
- B01L 2200/12: Specific details about manufacturing devices
- B01L 2200/142: Preventing evaporation
- B01L 2300/041: Connecting closures to device or container
- B01L 2300/044: pierceable, e.g. films, membranes
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/0848: Specific forms of parts of containers
- B01L 2300/12: Specific details about materials
- G01N 35/028: {having reaction cells in the form of microtitration plates}
- G01N 2035/00277: {Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to a micro chamber plate, and more particularly, to a micro chamber plate having micro chamber holes formed using a flowable film. Thus, samples can be injected into the micro chamber holes in a smoother manner compared to when samples are injected using a vacuum and/or centrifugal force. In addition, bubbles and excess samples in the micro chamber holes can be efficiently discharged, making it possible to perform reaction and analysis in a more accurate and efficient manner.

## Claims

### Claim 1

A method for manufacturing a micro chamber plate, the method comprising the steps of:
(a) attaching a flowable film to a top side of a micro chamber body;
(b) blow molding to attach the flowable film to a lateral side of each micro chamber hole and, at the same time, to form the flowable film at a bottom side of the micro chamber hole, thereby forming micro chamber holes; and
(c) welding a micro chamber body covering part to the flowable film on the top side of the micro chamber body.

### Claim 2 (depends on 1)

The method of claim 1, further comprising a step (b) of injecting a specific component for analyzing biological substances into each micro chamber hole, between steps (b) and (c).

### Claim 3 (depends on 1)

The method of claim 1, wherein the blow molding in the step (b) is performed at a temperature of 100° C. to 300° C.

### Claim 4 (depends on 1)

The method of claim 1, wherein the micro chamber plate of claim 1, wherein in step (a), an adhesive film having an adhesive property is further provided between the flowable film and the micro chamber body or between the flowable film and the micro chamber body covering part.

### Claim 5 (depends on 1)

The method of claim 1, wherein the flowable film is transparent such that it allows optical measurement.

### Claim 6 (depends on 5)

The method of claim 5, wherein the flowable film is made of a material selected from among polyethylene, polyethylene terephthalate, stretched or non-stretched polypropylene, polycarbonate, PMMA, and a copolymer thereof.

### Claim 7 (depends on 1)

The method of claim 1, wherein the micro chamber body covering part has formed therein at least one sample inlet per micro chamber hole.

### Claim 8 (depends on 1)

The method of claim 1, wherein the micro chamber body covering part is made of a metal or a polymer film.

### Claim 9 (depends on 1)

The method of claim 1, wherein the micro chamber body covering part is made of a porous membrane having a pore size of 0.2-5.0 μm.

### Claim 10 (depends on 1)

The method of claim 1, wherein each sample inlet has an area of 0.05-60% based on total area of each micro chamber hole.

### Claim 11 (depends on 1)

The method of claim 1, wherein in step (c), the micro chamber body covering part is bonded to the flowable film by at least one method selected from thermal bonding, ultrasonic bonding, and adhesive agent coating.

### Claim 12 (depends on 1)

The method of claim 1, wherein the surface of the micro chamber body is coated with at least one material selected from a polyester-based polymer resin and a polyethylene-based hot-melt adhesive.

### Claim 13 (depends on 1)

The method of claim 1, further comprising steps of: (d) injecting the sample; and (e) sealing the sample inlet, after the step (c).

### Claim 14 (depends on 13)

The method of claim 13, wherein the sealing of the sample inlet is performed using at least one material selected from the group consisting of a polymer film, oil, silicone, paraffin and an adhesive agent.