# US 2021/0274802 A1 · DHA Enriched Polyunsaturated Fatty Acid Compositions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0274802 A1 - Document ID: US20210274802A1 - Status: Published application - Kind: A1 - Application number: 17/253,568 - Filed: 2018-12-20 - Published: 2021-09-09 - Earliest claimed: 2018-06-21 - Assignees: Nuseed Pty Ltd - Applicants: Nuseed Pty Ltd. - Inventors: Stuart LITTLER - Web page: https://patentgrep.com/patent/US20210274802A1 ## CPC - A23D 7/0053: {Compositions other than spreads} - A23D 9/00: Other edible oils or fats, e.g. shortenings or cooking oils (preservation of other edible oils A23B20/30) - A23D 7/04: Working-up - A23D 9/02: characterised by the production or working-up - C11B 3/12: by distillation - A23D: EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS (preservation thereof A23B20/00; production, refinement or preservation of animal or vegetable fats or oils C11B, C11C) - C11B: PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES (drying-oils C09F) ## Abstract There is provided a vegetable-based lipid composition comprising high levels of at least three different long-chain polyunsaturated fatty acids (typically as fatty acid esters). The compositions typically contain DHA as the principal long-chain polyunsaturated fatty acid. The composition is obtainable from a single source by conventional processing methods. and has improved stability properties. ## Claims ### Claim 1 A vegetable-based lipid composition comprising: (i) a first polyunsaturated fatty acid in an amount of from about 50% to about 85% by weight of the total fatty acid content of the composition; wherein the first polyunsaturated fatty acid has at least five unsaturations in its carbon chain, has at least 20 carbon atoms in its carbon chain, or is selected from the group consisting of docosahexaeneoic acid (DHA) and eicosapentaenoic (EPA); (ii) α-linolenic acid (ALA) in an amount of from about 10% to about 90% by weight of the first polyunsaturated fatty acid; and (iii) a third polyunsaturated fatty acid in an amount of from about 10% to about 70% by weight of the ALA; wherein the total amount of all other fatty acids in the composition is up to about 20% by weight of the total fatty acid content of the composition, and wherein each of the ALA, the first and the third polyunsaturated fatty acids is independently provided in the form of a fatty acid, a fatty acid salt, a fatty acid ester or a salt of a fatty acid ester. ### Claim 2 (depends on 1) The lipid composition of claim 1, wherein the first polyunsaturated fatty acid is present in an amount that is at least 55% by weight of the total fatty acid content of the composition. ### Claim 3 (depends on 1) The lipid composition of claim 1, wherein the ALA is present in an amount of from about 10% to about 60% by weight of the first polyunsaturated fatty acid. ### Claim 4 (depends on 1) The lipid composition of claim 1, wherein the third polyunsaturated fatty acid is present in an amount of from about 10% to about 65% by weight of the ALA. ### Claim 5 (depends on 1) The lipid composition claim 1, wherein the first polyunsaturated fatty acid is DHA, docosapentaenoic acid (22:5n-3) (DPA), or EPA. ### Claim 6 (depends on 1) The lipid composition of claim 1, wherein the third polyunsaturated fatty acid is a C:18-24 omega-3 polyunsaturated fatty acid containing at least three unsaturations. ### Claim 7 (depends on 1) The lipid composition of claim 1, wherein the ALA and the first and third polyunsaturated fatty acids are each independently provided in the form of a fatty acid ester or a salt of a fatty acid ester, such as in the form of a fatty acid ethyl ester or as part of a triglyceride. ### Claim 8 (depends on 1) The lipid composition of claim 1, wherein the lipid composition is derived from a single source. ### Claim 9 (depends on 1) The lipid composition of claim 1, wherein the lipid composition is derived from a plant. ### Claim 10 (depends on 9) The lipid composition of claim 9, wherein the plant-based oil is from an oilseed selected from Brassica sp., Gossypium hirsutum, Linum usitatissimum, Helianthus sp., Carthamus tinctorius, Glycine max, Zea mays, Arabidopsis thaliana, Sorghum bicolor, Sorghum vulgare, Avena saliva, Trifolium sp., Elaesis guineensis, Nicotiana benthamiana, Hordeum vulgare, Lupinus angustifolius, Oryza saliva, Oryza glaberrima, Camelina saliva, or Crambe abyssinica. ### Claim 11 (depends on 1) The lipid composition of claim 1, wherein the composition is provided in the form of a tablet, capsule, encapsulated gel, ingestible liquid or powder, emulsion, or a topical ointment or cream. ### Claim 12 (depends on 1) The lipid composition of claim 1, further comprising one or more additional components selected from the group consisting of an antioxidant, a stabilizer, and a surfactant. ### Claim 13 (depends on 1) A nutraceutical composition comprising the lipid composition of claim 1. ### Claim 14 (depends on 1) A food product for human or animal consumption comprising the lipid composition of claim 1, wherein the ALA and the first and third polyunsaturated fatty acids are provided in the form of triglyceride esters. ### Claim 15 (depends on 1) The vegetable-based lipid composition of claim 1 for use in the treatment or prevention of cardiovascular disease, protection against death in patients with cardiovascular disease, reduction of overall serum cholesterol levels, reduction in high blood pressure, increase in HDL:LDL ratio, reduction of triglycerides, or reduction of apolipoprotein-B levels. ### Claim 16 (depends on 1) A method for producing a vegetable-based lipid composition of claim 1, comprising providing a mixture of fatty acid ethyl esters, and subjecting said mixture to a chromatographic separation process, optionally wherein the mixture of fatty acid ethyl esters is obtained by transesterification and distillation of a vegetable-based lipid oil. ### Claim 17 (depends on 2) The lipid composition of claim 2, wherein the first polyunsaturated fatty acid is present in an amount that is at least 60% by weight of the total fatty acid content of the composition. ### Claim 18 (depends on 6) The lipid composition of claim 6, wherein the third polyunsaturated fatty acid is selected from the group consisting of DHA, EPA, DPA, eicosatetraenoic acid (20:4n-3) (ETA), and stearadonic acid (18:4n-3).