# US 2021/0274791 A1 · Compositions and methods to treat citrus greening disease

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- Number: US 2021/0274791 A1
- Document ID: US20210274791A1
- Status: Published application
- Kind: A1
- Application number: 16/325,120
- Filed: 2017-05-18
- Published: 2021-09-09
- Earliest claimed: 2016-10-31
- Assignees: Marrone Bio Innovations, Inc.
- Applicants: Marrone Bio Innovations, Inc.
- Inventors: Pamela G. Marrone
- Web page: https://patentgrep.com/patent/US20210274791A1

## CPC

- A01G 7/06: Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
- A01N 25/06: Aerosols
- A01N 25/14: wettable
- A01N 25/30: characterised by the surfactants
- A01N 63/34: Aspergillus
- A01N 63/38: Trichoderma
- A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The present disclosure includes the field of agricultural biologicals, and provides microorganism compositions for preventing and controlling citrus huanglongbing and their methods of use thereof.

## Claims

### Claim 1

A citrus greening disease control fungal composition comprising (i) a whole cell broth or filtrant collected from Aspergillus ochraceus, Trichoderma virens or Trichoderma harzianum fermentation; and (ii) a carrier, diluent, adjuvant or surfactant.

### Claim 2

A citrus greening disease control fungal composition comprising (i) a whole cell broth or filtrant collected from Aspergillus ochraceus (ATCC 18412), Trichoderma virens S101 (NRRL 67411), Trichoderma virens S102 (NRRL 67412), Trichoderma virens S103 (NRRL 67413), Trichoderma virens S090 (NRRL 67414) or Trichoderma harzianum S117 (NRRL 67415) fermentation; and (ii) a carrier, diluent, adjuvant or surfactant.

### Claim 3 (depends on 1)

A plant or plant parts comprising the composition of claim 1.

### Claim 4 (depends on 1)

The composition of claim 1, wherein said fungal strain is present in a formulation selected from the group consisting of an emulsifiable concentrate, a wettable powder, a soluble liquid, an aerosol, an ultra-low volume concentrate solution, a soluble powder, a microencapsulate, water-dispersed granules, a flowable, a micro emulsion and a nano-emulsion.

### Claim 5 (depends on 1)

The composition of claim 1, further comprising a plant fertilizer or one or more reagent having nematicidal, fungicidal or insecticidal activity.

### Claim 6 (depends on 1)

A method to control citrus greening disease in a plant comprising:
applying to the plant, plant parts, seed and/or substrate from the plant, an amount of composition of claim 1, effective to control said citrus greening disease.

### Claim 7 (depends on 6)

The method according to claim 6, further comprising transplanting said plant into said growth substrate.

### Claim 8 (depends on 6)

The method of claim 6, wherein said plant is a citrus plant.

### Claim 9 (depends on 8)

The method of claim 8, wherein said citrus is selected from the group consisting of Citrus maxima (Pomelo); Citrus medica (Citron); Citrus micrantha (Papeda); Citrus reticulata (Mandarin orange); Citrus trifolata (trifoliate orange); Citrus japonica (kumquat); Citrus australasica (Australian Finger Lime); Citrus australis (Australian Round lime); Citrus glauca (Australian Desert Lime); Citrus garrawayae (Mount White Lime); Citrus gracilis (Kakadu Lime or Humpty Doo Lime); Citrus inodora (Russel River Lime); Citrus warburgiana (New Guinea Wild Lime); Citrus wintersii (Brown River Finger Lime); Citrus halimii (limau kadangsa, limau kedut kera) Citrus indica (Indian wild orange); Citrus macroptera; and Citrus latipes; Citrus.times.aurantiifolia (Key lime); Citrus.times.aurantium (Bitter orange); Citrus.times.latifolia (Persian lime); Citrus.times.limon (Lemon); Citrus.times.limonia (Rangpur);Citrus.times.paradisi (Grapefruit), Citrus.times. sinensis (Sweet orange), Citrus.times.tangerina (Tangerine), Poncirus trifoliata.times.C. sinensis (Carrizo citrange), C. paradisi “Duncan” grapefruit.times.Pondirus trifoliate (Swingle citrumelo), Imperial lemon, tangelo, orangelo, tangor, kinnow, kiyomi, Minneola tangelo, oroblanco, sweet orange, ugli, Buddha's hand, citron, lemon, orange, bergamot orange, bitter orange, blood orange, calamondin, clementine, grapefruit, Meyer lemon; Rangpur; tangerine; and yuzu.

### Claim 10 (depends on 5)

A method to control citrus greening disease in a plant comprising:
applying to the plant, plant parts, seed and/or substrate from the plant, an amount of composition of claim 5, effective to control said citrus greening disease.

### Claim 11 (depends on 10)

The method according to claim 10, further comprising transplanting said plant into said growth substrate.

### Claim 12

A substantially purified microorganism comprising Aspergillus ochraceus (ATCC 18412), Trichoderma virens S101 (NRRL 67411), Trichoderma virens S102 (NRRL 67412), Trichoderma virens S103 (NRRL 67413), Trichoderma virens S090 (NRRL 67414) or Trichoderma harzianum S117 (NRRL 67415).