# US 2021/0274790 A1 · Methods of multi-species insect pest control

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- Number: US 2021/0274790 A1
- Document ID: US20210274790A1
- Status: Published application
- Kind: A1
- Application number: 17/192,764
- Filed: 2021-03-04
- Published: 2021-09-09
- Earliest claimed: 2020-03-05
- Assignees: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
- Applicants: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
- Inventors: Kwang-Zin Lee; Andreas Vilcinskas
- Web page: https://patentgrep.com/patent/US20210274790A1

## CPC

- A01N 63/32: Yeast
- C12N 1/16: Yeasts; Culture media therefor
- C12N 1/145: Fungi isolates
- C12N 1/165: Yeast isolates
- C12R 2001/84: Pichia
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Multi-species pest control methods, which contact or subject a target pest to a yeast strain belonging to the species Pichia Kluyveri and/or with a lysate, filtrate, isolate or extract thereof. The yeast strain belonging to the species Pichia Kluyveri and/or the lysate, filtrate, isolate or extract thereof is incorporated into the body of the agricultural target pest.

## Claims

### Claim 1

A pest control method for reducing the survival of a target pest, the method comprising contacting a target pest with a yeast strain belonging to the species Pichia Kluyveri and/or with a lysate, filtrate, isolate or extract thereof.

### Claim 2 (depends on 1)

The pest control method according to claim 1, wherein the target pest is an insect belonging to the order Hemiptera, optionally wherein the target pest belongs to the suborder Sternorrhyncha and/or to the infraorder Fulgoromorpha, optionally wherein the target pest belongs to the family of aphids like Acyrthosiphon pisum.

### Claim 3 (depends on 1)

The pest control method according to claim 1, wherein the target pest is an insect belonging to the family Drosophilidae, optionally wherein the target pest belongs to the genus Drosophila, optionally wherein the target pest belongs to the species of Drosophila suzukii and/or Drosophila melanogaster.

### Claim 4 (depends on 1)

The pest control method according to claim 1, wherein the yeast strain belonging to the species Pichia Kluyveri and/or a lysate, filtrate, isolate or extract thereof is comprised in a composition in combination with a carrier.

### Claim 5 (depends on 1)

The pest control method according to claim 1, wherein the yeast strain belonging to the species Pichia Kluyveri and/or a lysate, filtrate, isolate or extract thereof is orally led to the target pest and/or sprayed on a crop or part of a crop for contacting the target pest.

### Claim 6 (depends on 1)

The pest control method according to claim 1, further comprising spraying, or atomization of a crop in advance of the contact for incorporating the composition into the body of the target pest by ingestion of the crop or a part thereof.

### Claim 7 (depends on 1)

The pest control method according to claim 1, wherein the yeast strain belonging to the species Pichia Kluyveri can be identified with the ITS primers SEQ ID NO: 1 and SEQ ID NO: 2, or homologs thereof.

### Claim 8 (depends on 1)

The pest control method according to claim 1, wherein the yeast strain belonging to the species Pichia Kluyveri comprises an ITS sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7.

### Claim 9 (depends on 1)

The pest control method according to claim 1, which reduces the survival of the target pest, wherein the yeast strain belonging to the species Pichia Kluyveri is selected from the group consisting of Pichia Kluyveri EPY-1 (DSMZ Accession number 33403), yeast strains derived therefrom, and progenies or mutants thereof, wherein the mutants retain the properties of Pichia Kluyveri EPY-1.

### Claim 10

An isolated strain of the species Pichia Kluyveri having a deposit accession number DSMZ 33403, yeast strains derived therefrom, or progenies or mutants thereof, wherein the mutants thereof retain the properties of said isolated strain.

### Claim 11 (depends on 10)

A lysate, filtrate, isolate or extract comprising the isolated strain according to claim 10.

### Claim 12 (depends on 10)

A composition comprising a carrier and a yeast strain belonging to the species Pichia Kluyveri and/or a lysate, filtrate, isolate or extract thereof as a pest control agent against a target pest, wherein the strain is a yeast strain according to claim 10.