# US 2021/0274733 A1 · RESISTANCE TO ToLCNDV IN SQUASH

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- Number: US 2021/0274733 A1
- Document ID: US20210274733A1
- Status: Published application
- Kind: A1
- Application number: 17/323,439
- Filed: 2021-05-18
- Published: 2021-09-09
- Earliest claimed: 2015-11-10
- Assignees: Vilmorin & Cie
- Applicants: Vilmorin & Cie
- Inventors: Julien MAZET; Juan AGUILAR; Isabelle JUSTAFRE; Eric LIONNETON
- Web page: https://patentgrep.com/patent/US20210274733A1

## CPC

- A01H 1/126: {for virus resistance}
- A01H 1/045: {using molecular markers}
- A01H 5/08: Fruits
- A01H 5/10: Seeds
- A01H 6/348: {Cucurbita, e.g. squash or pumpkin}
- C12Q 1/6895: for plants, fungi or algae
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention provides a Tomato Leaf Curl New Dehli Virus (ToLCNDV) tolerance or resistant QTL in cucurbit plants and plants comprising the QTL. The invention also provides molecular markers linked to QTL. The invention further provides methods of breeding to produce plants that are resistant to ToLCNDV, and the resistant plants produced by such methods.

## Claims

### Claim 1

A Cucurbita pepo (C. pepo) plant tolerant or resistant to Tomato Leaf Curl New Dehli Virus (ToLCNDV), wherein said plant comprises one QTL associated to tolerance or resistance to ToLCNDV on linkage group 11 (LG11).

### Claim 2 (depends on 1)

The C. pepo plant according to claim 1, wherein said QTL on LG11 is located in a locus encompassing the markers SQ-0006678 (SEQ ID NO: 8), SQ-0010844 (SEQ ID NO: 9) and/or SQ-0000209 (SEQ ID NO: 10).

### Claim 3 (depends on 1, 2)

The C. pepo plant according to claim 1 or 2, wherein said QTL associated to tolerance or resistance to ToLCNDV has been introgressed from a Cucurbita moschata (C. moschata) plant.

### Claim 4 (depends on 1, 2, 3)

The C. pepo plant according to any one of claims 1 to 3, wherein said QTL associated to tolerance or resistance to ToLCNDV is chosen from those present in the genome of the plant TLG, a representative sample of seed of which has been respectively deposited under NCIMB number 42686.

### Claim 5 (depends on 1, 2, 3, 4)

The C. pepo plant according to any one of claims 1 to 4, wherein said plant is a progeny of a plant TLG (NCIMB accession number 42686).

### Claim 6 (depends on 1, 2, 3, 4, 5)

A cell of a C. pepo plant according to any one of claims 1 to 5.

### Claim 7 (depends on 1, 2, 3, 4, 5)

A plant part obtained from a C. pepo plant according to any one of claims 1 to 5.

### Claim 8 (depends on 1, 2, 3, 4, 5)

A seed of a C. pepo plant, giving rise when grown up to a plant according to any one of claims 1 to 5.

### Claim 9

A method of breeding a plant of the genus Cucurbita tolerant or resistant to Tomato Leaf Curl New Dehli Virus (ToLCNDV), wherein the method comprises crossing a first cucurbit plant of the genus Cucurbita with a second plant of the genus Cucurbita to produce progeny plants of a subsequent generation, and selecting one or more progeny plants that contain the tolerance or resistance to ToLCNDV from the progeny of the subsequent generation, wherein the first plant of the genus Cucurbita is tolerant or resistant to ToLCNDV.

### Claim 10 (depends on 9)

The method according to claim 9, wherein the method further comprises:
(i) backcrossing the selected progeny plants that contain the tolerance or resistance to ToLCNDV or selfed offspring thereof with the second cucurbit plant to produce backcross progeny plants,
(ii) selecting for backcross progeny plants that contain the tolerance or resistance to ToLCNDV from the backcross progeny plants, and
(iii) repeating steps (i) and (ii) three or more times in succession to produce selected fourth or higher backcross progeny plants that are tolerant or resistant to ToLCNDV,
wherein the progeny plants selected in (ii) and (iii) are optionally selected by detecting the presence of a QTL associated to tolerance or resistance to ToLCNDV as indicated by the presence of one or more of the markers SQ-0006678 (SEQ ID NO: 8), SQ-0010844 (SEQ ID NO: 9) and/or SQ-0000209 (SEQ ID NO: 10).

### Claim 11 (depends on 9, 10)

The method according to claim 9 or 10, wherein said first plant of the genus Cucurbita resistant to ToLCNDV is a C. moschata plant or a C. pedatifolia plant.

### Claim 12 (depends on 11)

The method according to claim 11, wherein said C. moschata plant is ‘Nigerian’, ‘Menina Portugal’, ‘Menina Brasileira’, or hybrid thereof.

### Claim 13 (depends on 9, 10, 11, 12)

The method according to any one of claims 9 to 12, wherein said second plant of the genus Cucurbita is a C. pepo plant.

### Claim 14 (depends on 9, 10, 11, 12, 13)

A plant of the genus Cucurbita produced by the method according to any one of claims 9 to 13, wherein said plant is tolerant or resistant to ToLCNDV.

### Claim 15 (depends on 14)

A plant, plant part, or plant cell derived from the plant of claim 14.

### Claim 16 (depends on 15)

A seed produced by the plant of claim 15.