# US 2021/0274732 A1 · S-methylmethionine-rich brassica oleracea plant

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- Number: US 2021/0274732 A1
- Document ID: US20210274732A1
- Status: Published application
- Kind: A1
- Application number: 17/258,020
- Filed: 2019-07-09
- Published: 2021-09-09
- Earliest claimed: 2018-07-10
- Assignees: Sakata Seed Corporation
- Applicants: Sakata Seed Corporation
- Inventors: Manabu KAWAMURA
- Web page: https://patentgrep.com/patent/US20210274732A1

## CPC

- A01H 1/108: {involving amino acid content, e.g. synthetic storage proteins or altering amino acid biosynthesis}
- A61K 36/31: Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A01H 5/10: Seeds
- A01H 6/203: {Brassica oleraceae, e.g. broccoli or kohlrabi}
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A23L 33/175: Amino acids
- A61K 31/198: Alpha-amino acids, e.g. alanine or edetic acid [EDTA] (betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00)
- A61P 1/04: for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
- C07C 319/28: Separation; Purification
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07C: ACYCLIC OR CARBOCYCLIC COMPOUNDS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

This invention provides a Brassica oleracea plant with high S-methylmethionine content. This invention relates to a Brassica oleracea plant with higher S-methylmethionine content than that in a conventional Brassica oleracea plant. Specifically, this invention relates to a Brassica oleracea plant with the S-methylmethionine content of 48 mg/100 g fresh weight or more.

## Claims

### Claim 1

A Brassica oleracea plant comprising S-methylmethionine in a content of 48 mg/100 g fresh weight (FW) or more.

### Claim 2

A Brassica oleracea plant having a genetic trait causing an increased content of S-methylmethionine in a plant, the genetic trait being present in a broccoli, the representative seeds of which are deposited under Accession Number: FERM BP-22352, or in a progeny of the broccoli having a genetic trait causing an increased content of S-methylmethionine in a plant.

### Claim 3 (depends on 1)

The Brassica oleracea plant according to claim 1, which is selected from the group consisting of broccoli, cauliflower, Chinese kale, cabbage, Brussels sprouts, kohlrabi, kale, and a hybrid thereof.

### Claim 4 (depends on 3)

The Brassica oleracea plant according to claim 3, which is broccoli.

### Claim 5 (depends on 1)

A part of the Brassica oleracea plant according to claim 1.

### Claim 6 (depends on 1)

A seed of the Brassica oleracea plant according to claim 1.

### Claim 7 (depends on 1)

A method for producing a Brassica oleracea plant comprising a step of cross pollinating the Brassica oleracea plant according to claim 1 with another Brassica oleracea plant.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the Brassica oleracea plant comprises S-methylmethionine in a content of 48 mg/100 g FW or more.

### Claim 9 (depends on 7)

The method according to claim 7, which further comprises a step of producing doubled haploids from the progeny plants obtained in the step of cross pollinating via anther culture or pollen culture.

### Claim 10 (depends on 1)

A Brassica oleracea plant, a seed of the plant, or a part of the plant, the plant being obtainable by a method for producing a Brassica oleracea plant comprising a step of cross pollinating the Brassica oleracea plant according to claim 1 with another Brassica oleracea plant.

### Claim 11 (depends on 10)

The Brassica oleracea plant, the seed of the plant, or the part of the plant according to claim 10, comprising S-methylmethionine in a content of 48 mg/100 g FW or more.

### Claim 12

A method for producing a Brassica oleracea plant comprising S-methylmethionine in a content of 48 mg/100 g FW or more comprising:
a step of cross pollinating Brassica oleracea plants;
a step of producing doubled haploids from a progeny plant obtained in the step of cross pollinating via anther culture or pollen culture; and
a step of selecting a Brassica oleracea plant comprising S-methylmethionine in a content of 48 mg/100 g FW or more from among the doubled haploids produced by the step of producing doubled haploids.

### Claim 13 (depends on 1)

An S-methylmethionine-containing composition, comprising the Brassica oleracea plant according to claim 1, a part of the plant, or a processed product of the plant or the part.

### Claim 14 (depends on 1)

A method for producing S-methylmethionine, comprising obtaining S-methylmethionine from the Brassica oleracea plant according to claim 1, a part of the plant, or a processed product of the plant or the part.

### Claim 15

(canceled)

### Claim 16 (depends on 2)

A method for producing a Brassica oleracea plant comprising a step of cross pollinating the Brassica oleracea plant according to claim 2 with another Brassica oleracea plant.

### Claim 17 (depends on 2)

A Brassica oleracea plant, a seed of the plant, or a part of the plant, the plant being obtainable by a method for producing a Brassica oleracea plant comprising a step of cross pollinating the Brassica oleracea plant according to claim 2 with another Brassica oleracea plant.

### Claim 18 (depends on 2)

An S-methylmethionine-containing composition, comprising the Brassica oleracea plant according to claim 2, a part of the plant, or a processed product of the plant or the part.

### Claim 19 (depends on 2)

A method for producing S-methylmethionine, comprising obtaining S-methylmethionine from the Brassica oleracea plant according to claim 2, a part of the plant, or a processed product of the plant or the part.
