Composition for diagnosing diseases associated with cox2 overexpression and screening method therefor
AssigneeKyungpook National University Industry-Academic Cooperation Foundation
InventorsJae-Sung BAE, Hee Kyung JIN, Ju Youn LEE
Life of this patent
Estimating the term…
Abstract
The present invention relates to a composition for diagnosing diseases associated with cyclooxygenase 2 (COX2) overexpression and a screening method therefor and, more particularly, to: a composition for diagnosing diseases associated with COX2 overexpression, containing a compound with a structural feature of exhibiting strong binding activity to a COX2 protein; and a screening method therefor.
Claims19
A composition for diagnosing diseases associated with COX2 overexpression comprising a compound containing a functional group that interacts with at least one amino acid selected from the group consisting of N181, T564, S567, and S565 of a cyclooxygenase 2 (COX2) protein defined by SEQ ID NO:
- 1.
A screening method for a diagnostic substance of diseases associated with COX2 overexpression comprising:
- (a) contacting a test substance with a COX2 protein defined by SEQ ID NO:
- 1;
- (b) measuring whether the test substance interacts with at least one amino acid selected from the group consisting of N181, T564, S567, and S565 of the COX2 protein defined by SEQ ID NO:
- 1;
- and
- (c) selecting a substance that interacts with the COX2 protein in step (b).
(canceled)
A method for diagnosing diseases associated with COX2 overexpression, the method comprising:
- a) obtaining a biological sample from a subject suspected of having the diseases associated with COX2 overexpression;
- b) administering a compound containing a functional group that interacts with at least one amino acid selected from the group consisting of N181, T564, 5567, and S565 of a cyclooxygenase 2 (COX2) protein defined by SEQ ID NO:
- 1 to the sample;
- c) measuring whether the administered compound interacts with the COX2 protein in the sample in step b);
- and
- d) comparing the degree of interaction between the COX2 protein and the compound in comparison with that of a normal control group, and diagnosing the subject as having the diseases associated with COX2 overexpression when the degree of interaction between the COX2 protein and the compound is increased as compared with that of the normal control group.
Dependencies are inferred from the claim text.