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US 2021/0268489 A1Published application
Multiplexed polymerase chain reaction in micropipette format
OwnerAndrew Brodskyinventor
Life of this patent
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Abstract
A system may include a horizontal actuator to move a tray, to which a microwell plate and a microfluidic chip may be coupled. The system may include a vertical actuator to move a support arm, to which a plurality of pipettes or pipette tips may be coupled. The system may include a rotational actuator to move an angle bracket, to which a magnet may be coupled. The system may include a heater, through which the pipettes may extend. The system may include a pump to control the flow of fluids through the pipettes. Disclosed methods include performing PCR within the described system.
Claims20
A method, comprising:
- operating a pump to draw a biological sample from a well of a well plate into a pipette through a pipette tip of the pipette;
- operating a first actuator to move a first valve to a closed position to seal the biological sample within the pipette from the pipette tip;
- operating the pump to apply a positive gage pressure to the biological sample within the pipette;
- operating a second actuator to move a second valve to a closed position to seal the biological sample within the pipette from the pump;
- and
- operating a heater to heat the biological sample within the pipette, wherein a polymerase chain reaction occurs within the heated biological sample within the pipette between the first valve and the second valve.
3 more elements
A method, comprising:
- operating a pump to draw a biological sample from a well of a well plate into a first pipette conduit through a first pipette tip;
- operating a first actuator to move a first valve to a closed position to seal the biological sample within the first pipette conduit from the first pipette tip;
- operating the pump to apply a positive gage pressure to the biological sample within the first pipette conduit;
- operating a second actuator to move a second valve to a closed position to seal the biological sample within the first pipette conduit from the pump;
- and
- operating a heater to heat the biological sample within the first pipette conduit, wherein a chemical reaction occurs within the heated biological sample within the first pipette conduit between the first valve and the second valve.
3 more elements
A system, comprising:
- a first pipette including a first pipette tip and a first end of the first pipette opposite to the first pipette tip along a first length of the first pipette;
- a second pipette including a second pipette tip and a second end of the second pipette opposite to the second pipette tip along a second length of the second pipette;
- a three-way connector that fluidically couples the second end of the first pipette to the second end of the second pipette and fluidically couples the second ends of the first and second pipettes to a feeder conduit;
- and
- a heating element thermally coupled to at least a portion of the first and second lengths.
2 more elements
Dependencies are inferred from the claim text.