Malaria vaccine and methods for producing same
AssigneesThe Walter and Eliza Hall Institute of Medical Research, Expres2ion Biotechnologies
InventorsAlan Cowman, Julie Healer, Willem Adriaan De Jongh, Teit Max Moscote Sogaard, Thomas Dan Jorgensen, Vladyslav Soroka
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Abstract
The present description relates to malaria vaccines comprising Plasmodium falciparum (Pf) polypeptide complexes and methods of producing the same. The Pf polypeptides in complexes or in a partially complexed arrangement may comprise two or more of the following polypeptides: PfRipr, PfCyrPa and PfRh5. Drosophila cells and expression vectors are also described.
Claims19
-24. (canceled)
A Drosophila derived cell modified to express a Plasmodium falciparum (Pf) Ripr polypeptide from a heterologous polynucleotide encoding a PfRipr polypeptide and a suitable signal sequence, further comprising a heterologous polynucleotide encoding PfRh5 polypeptide and a suitable signal sequence, wherein each polynucleotide is operably linked to an expression control sequence which induces the Pf polypeptide expression in the cell and wherein the PfRipr polypeptide and the PfRh5 polypeptide from said cell induce merozoite invasion inhibitory antibodies when administered to a subject.
A Drosophila cell comprising an expression vector comprising a polynucleotide encoding PfRipr polypeptide and a suitable signal sequence and an expression vector comprising a polynucleotide encoding PfRh5 polypeptide and a suitable signal sequence, wherein each polynucleotide is operably linked to an expression control sequence which induces Pf Ripr and PfRh5 polypeptide expression in a Drosophila derived cell, wherein the PfRipr polypeptide and the PfRh5 polypeptide from the cell induces parasite growth inhibitory antibodies when administered to a subject.
An expression vector comprising a polynucleotide encoding PfRipr polypeptide and a suitable signal sequence and a polynucleotide encoding PfRh5 polypeptide and a suitable signal sequence, wherein each polynucleotide is operably linked to an expression control sequence which induces PfRipr and PfRh5 polypeptide expression in a Drosophila derived cell, wherein the PfRipr polypeptide and the PfRh5 polypeptide from the cell induces parasite growth inhibitory antibodies when administered to a subject.
An immunogenic composition comprising an expression vector comprising a polynucleotide encoding PfRipr polypeptide and a suitable signal sequence and an expression vector comprising a polynucleotide encoding PfRh5 polypeptide and a suitable signal sequence, wherein each polynucleotide is operably linked to an expression control sequence which induces PfRipr and PfRh5 polypeptide expression, and a physiologically or pharmaceutically acceptable carrier, diluent or excipient.
Dependencies are inferred from the claim text.