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US 2021/0268033 A1Published application
Flowable birth tissue composition and related methods
AssigneeTriad Life Sciences, Inc.
InventorsKurt KLITZKE, Jon G. HARGIS
Life of this patent
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Abstract
A flowable birth tissue composition fabricated from birth tissue is provided. Methods of processing a mammal's placental tissue to form a flowable birth tissue composition are provided. Various methods of treatment and uses are also provided.
Claims20
A method of preparing a flowable porcine birth tissue composition, comprising the steps of:
- introducing from about 1 mL to about 40 mL of from about 1M to about 4M sodium chloride solution per gram of porcine placental tissue to the porcine placental tissue;
- decanting the sodium chloride;
- rinsing the sodium chloride from the placental tissue with water;
- introducing from about 5 mL to about 25 mL of a detergent solution per gram of placental tissue to the porcine placental tissue;
- decanting the detergent solution;
- rinsing the detergent solution from the porcine placental tissue with water;
- introducing from about 5 mL to about 15 mL of from about 0.1M to about 1.0M sodium hydroxide per gram of placental tissue to the porcine placental tissue;
- rinsing the sodium hydroxide from the porcine placental tissue with water;
- introducing from about 5 mL to about 50 mL of buffer solution per gram of porcine placental tissue to the porcine placental tissue;
- decanting the buffer solution;
- rinsing the buffer solution from the porcine placental tissue with water;
- milling the placental tissue to form a porcine placental tissue powder;
- and
- homogenizing a pharmaceutically-acceptable carrier with the porcine placental tissue powder to form a flowable porcine birth tissue composition.
11 more elements
A method of preparing a flowable porcine birth tissue composition, comprising the steps of:
- introducing from about 1 mL to about 40 mL of from about 1M to about 4M sodium chloride solution per gram of porcine placental tissue to the porcine placental tissue;
- decanting the sodium chloride;
- rinsing the sodium chloride from the porcine placental tissue with water;
- introducing from about 5 mL to about 25 mL of a detergent solution per gram of porcine placental tissue to the porcine placental tissue;
- decanting the detergent solution;
- rinsing the detergent solution from the porcine placental tissue with water;
- introducing from about 5 mL to about 15 mL of from about 0.1M to about 1.0M sodium hydroxide per gram of porcine placental tissue to the porcine placental tissue;
- rinsing the sodium hydroxide from the porcine placental tissue with water;
- introducing from about 5 mL to about 50 mL of buffer solution per gram of porcine placental tissue to the porcine placental tissue;
- decanting the buffer solution;
- rinsing the buffer solution from the porcine placental tissue with water;
- milling the placental tissue to form a placental tissue powder;
- mixing hyaluronic acid and a radioprotective solution to form a suspension gel;
- and
- homogenizing the suspension gel with the porcine placental tissue powder to form a flowable porcine birth tissue composition.
12 more elements
A flowable porcine birth tissue composition comprising porcine placental tissue.
A flowable porcine birth tissue composition comprising:
- a porcine placental tissue treated with a bioburden reduction step, a detergent treatment step, and a viral inactivation step.
Dependencies are inferred from the claim text.