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US 2021/0268031 A1Published application
Cartilage Regeneration by Synovial Fluid-Derived Stem Cells and Their Derivatives
AssigneeTarachon LLC
InventorsTara Sharif, Vijay Vad
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Abstract
A method of making mammalian chondroblast cells or progenitors thereof and a method for their use. A method of making exosomes and signaling factors and a method for their use.
Claims22
A method of making mammalian chondroblast cells, or
- progenitors thereof, comprising:
- growing free-floating synovial fluid cells extracted from a subject's synovial cavity, resulting in proliferation and formation of an adherent cell culture containing one or more colonies of mesenchymal stem cells exhibiting CD90, CD73 and CD105 cell surface markers, wherein said colonies of the stem cells constitute at least about 88% of total cells in said cell culture;
- wherein said growing step is performed without any added growth and differentiation factors.
2-27. (canceled)
A method of making extracellular vesicles comprising:
- collecting extracellular vesicles by any of:
- synovial fluid mesenchyme stem cells (SF-MSCs) harvested from injured or healthy joint tissues;
- or
- chondroblast cells, or progenitors thereof, made from said synovial fluid mesenchyme stem cells (SF-MSCs);
- or
- mesenchyme stem cells or progenitor cells isolated from a source other than synovial fluid;
- wherein said chondroblast cells, or progenitors thereof, are made by a method comprising:
- harvesting free-floating synovial fluid cells from a subject's synovial cavity;
- allowing proliferation and formation of an adherent cell culture containing one or more colonies of mesenchymal stem cells exhibiting CD90, CD73 and CD105 cell surface markers, wherein said colonies of the stem cells constitute at least about 88% of total cells in said cell culture, wherein the temporal duration of the proliferation and formation of an adherent cell culture is in a range of about 2 weeks to about 3 weeks.
6 more elements
Dependencies are inferred from the claim text.