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US 2021/0267190 A1Published application
Cryopreservation
AssigneeNantKwest, Inc.
InventorsShannyn BESSETTE, Syed Raza ALI, Manju SAXENA
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Abstract
Provided herein are methods and medium compositions for cryopreserving NK-92® cells.
Claims37
A method of freezing NK-92®cells, the method comprising:
- contacting NK-92® cells with a cell freezing medium, wherein the cell freezing medium comprises a first composition and a second composition, wherein the first composition comprises:
- (a) one or more electrolytes selected from the group consisting of potassium ions, sodium ions, magnesium ions, and calcium ions;
- (b) one or more macromolecular agents selected from the group consisting of human albumin, polysaccharide and colloidal starch;
- (c) a pH buffer;
- (d) at least one sugar;
- (e) at least one sugar alcohol;
- (f) an impermeant agent selected from the group consisting of lactobionate, gluconate, citrate and glycerophosphate;
- and
- (g) DMSO;
- and wherein the second composition comprises albumin.
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A NK-92® cell culture comprising:
- NK-92® cells and a cell freezing medium, wherein the cell freezing medium comprises a first composition and a second composition, wherein the first composition comprises:
- (a) one or more electrolytes selected from the group consisting of potassium ions, sodium ions, magnesium ions, and calcium ions;
- (b) one or more macromolecular agents selected from the group consisting of human albumin, polysaccharide and colloidal starch;
- (c) a pH buffer;
- (d) at least one sugar;
- (e) at least one sugar alcohol;
- (f) an impermeant agent being at least one member selected from the group consisting of lactobionate, gluconate, citrate and glycerophosphate;
- and
- (g) DMSO;
- and
- wherein the second composition comprises albumin.
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A cell freezing medium produced by mixing a first composition and a second composition at a one to one ratio, wherein the first composition comprises:
- (a) one or more electrolytes selected from the group consisting of potassium ions, sodium ions, magnesium ions, and calcium ions;
- (b) one or more macromolecular agents selected from the group consisting of human albumin, polysaccharide and colloidal starch;
- (c) a pH buffer;
- (d) at least one sugar;
- (e) at least one sugar alcohol;
- (f) an impermeant agent selected from the group consisting of lactobionate, gluconate, citrate and glycerophosphate;
- and
- (g) DMSO;
- and wherein the second composition comprises albumin.
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Dependencies are inferred from the claim text.