Imaging mass spectrometry method
AssigneeSekisui Medical Co., Ltd.
InventorsMasashi HIRAMOTO, Toshimasa ITO
Life of this patent
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Abstract
A problem to be solved by the present invention to perform an imaging mass spectrometry method capable of not only providing localization information of a biological substance in a single tissue section but also enabling quantitative comparison of an amount of a biological substance between multiple tissue sections. By using a first derivatization reagent, a second derivatization reagent that is the isotopically-labeled first derivatization reagent, and a derivatized internal standard substance of a biological substance in the imaging mass spectrometry method, the amount of the biological substance can quantitatively be compared between multiple tissue sections.
Claims20
An imaging mass spectrometry method of a biological substance multiple sample sections, the method comprising the steps of:
- (A) bringing a first derivatization reagent into contact with the biological substance in a first sample section;
- (B) applying an internal standard substance of the biological substance to the first sample section, the internal standard substance being derivatized with a second derivatization reagent;
- (C) bringing the biological substance and the internal standard substance on the first sample section into contact with a matrix to obtain a mass spectrum;
- (D) bringing the first derivatization reagent or the second derivatization reagent into contact with the biological substance in a second sample section;
- (E) applying the internal standard substance of the biological substance to the second sample section, the internal standard substance being derivatized with the first derivatization reagent or the second derivatization reagent not used in the step (D);
- (F) bringing the biological substance and the internal standard substance on the second sample section into contact with the matrix to obtain the mass spectrum;
- and
- (G) quantitatively comparing a spectral intensity ratio of the derivatized biological substance and the derivatized internal standard substance between the first sample section and the second sample section,
- wherein the second derivatization reagent is the first derivatization reagent labeled with an isotope.
A quantification kit for a biological substance in a living body, comprising:
- a first derivatization reagent for labeling and derivatization of the biological substance;
- and
- an internal standard substance of the biological substance, which is derivatized with a second derivatization reagent,
- wherein an imaging mass spectrometry is used for quantification of the biological substance, and wherein the second derivatization reagent is the first derivatization reagent labeled with an isotope.
Dependencies are inferred from the claim text.