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US 2021/0262941 A1Published application
Multipartite luciferase peptides and polypeptides
AssigneePromega Corporation
InventorsVirginia Kincaid, Lance P. Encell, Mary Hall, Michael Killoran, Keith Wood, Melanie Dart, Trish Hoang
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Abstract
Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.
Claims22
-133. (canceled)
A system or kit comprising two or more peptide and/or polypeptide components collectively comprising 70% or greater sequence identity to SEQ ID NO:
- 788 or SEQ ID NO:
- 789;
- wherein the two or more peptide and/or polypeptide componets are capable of forming a bioluminescent complex upon interacting with each other;
- wherein a bioluminescent signal produced by the bioluminescent complex in the presence of a coelenterazine or a coelenterazine analog substrate is substantially increased when compared to a bioluminescent signal produced by the polypeptide or one or more complementary peptides and the coelenterazine substrate alone;
- wherein the system or kit comprises a polypeptide that corresponds structurally to 8 or fewer β strands of a Oplophorus gracilirostris luciferase.
2 more elements
-144. (canceled)
-192. (canceled)
Dependencies are inferred from the claim text.