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AssigneeCodiak BioSciences, Inc.
InventorsYoung Jun CHOI, Damian J. HOUDE, Delai CHEN, Douglas E. WILLIAMS
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Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of light scattering of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.
A method of detecting nanoparticles, comprising:
The method of any one of the preceding claims wherein said light scattering emission signal is determined at an excitation wavelength of 460 nm and an emission of 460 nm.
The method of any one of the preceding claims wherein the light scattering excitation signal is less than 519 nm or greater than 560 nm.
The method any one of the preceding claims, wherein said sample is separated into fractions prior to determining said light scattering emission signal.
The method of any one of the preceding claims, wherein said sample is subjected to a filtration step prior to determining said light scattering emission signal.
The method of any one of the preceding claims, wherein said sample is subjected to a centrifugation separation step prior to determining said light scattering emission signal.
The method of any one of the preceding claims, wherein said sample is subjected to a sucrose density gradient step prior to determining said light scattering emission signal.
The method of any one of the preceding claims, wherein said sample is a subjected to a separation step comprising use of a density gradient prior to determining said light scattering emission signal.
The method of any one of the preceding claims, wherein said sample is derived from a cell culture.
The method of any one of the preceding claims, wherein said sample is derived from a body fluid of an animal.
The method of any one of the preceding claims, comprising determining an amount of said nanoparticles within said sample comprising comparing said light scattering emission signal to the light scattering emission signal of a standard.
The method of any one of the preceding claims, comprising quantifying the amount of said nanoparticles within said sample, based upon the area under of the curve of a chromatogram comprising said light scattering emission signal.
The method of any one of the preceding claims, comprising determining an amount of said nanoparticles within said sample comprising comparing said light scattering emission signal to the luminescence signal of a standard.
The method of any of the above claims, wherein the nanoparticle is an extracellular vesicle.
The method of any of the above claims, wherein the nanoparticle is selected from the group comprising a ribosome, a polymeric bead, a metallic bead, a liposome, a lipid nanoparticle or combinations thereof.
A method of detecting nanoparticles in a sample, comprising:
Dependencies are inferred from the claim text.