Primer probe combination, kit, detection method and application for detecting separate candida types

A primer probe combination for detecting separate Candida types, comprising specific primers, detection probes and complementary probes specified at Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata respectively, wherein the complementary probes and the detection probes are in incomplete complementary pairing;

• nucleotide sequences of the specific primers of the Candida albicans are shown as SEQ ID NO.1-2;
• nucleotide sequences of the specific primers of the Candida tropicalis are shown as SEQ ID NO.3-4;
• nucleotide sequences of the specific primers of the Candida parapsilosis are shown as SEQ ID NO.5-6;
• nucleotide sequences of the specific primers of the Candida krusei are shown as SEQ ID NO.7-8;
• and nucleotide sequences of the specific primers of the Candida glabrata are shown as SEQ ID NO.9-10;
• a nucleotide sequence of the detection probe of the Candida albicans is shown as SEQ ID NO.11;
• a nucleotide sequence of the detection probe of the Candida tropicalis is shown as SEQ ID NO.12;
• a nucleotide sequence of the detection probe of the Candida parapsilosis is shown as SEQ ID NO.13;
• a nucleotide sequence of the detection probe of the Candida krusei is shown as SEQ ID NO.14;
• and a nucleotide sequence of the detection probe of the Candida glabrata is shown as SEQ ID NO.15;
• a nucleotide sequence of the complementary probe of the Candida albicans is shown as SEQ ID NO.16;
• a nucleotide sequence of the complementary probe of the Candida tropicalis is shown as SEQ ID NO.17;
• a nucleotide sequence of the complementary probe of the Candida parapsilosis is shown as SEQ ID NO.18;
• a nucleotide sequence of the complementary probe of the Candida krusei is shown as SEQ ID NO.19;
• and a nucleotide sequence of the complementary probe of the Candida glabrata is shown as SEQ ID NO.20.