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US 2021/0262011 A1Published application
Compositions and Methods for Analyte Detection
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Jehyuk Lee, Daniel Levner, Michael Super
Life of this patent
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Abstract
Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
Claims20
A method of making a three dimensional matrix including a plurality of nucleic acids comprising
- contacting a plurality of nucleic acids having a three-dimensional spatial relationship with a matrix-forming material in a manner to substantially retain the three-dimensional spatial relationship, and
- forming a three dimensional polymerized matrix including the plurality of nucleic acids covalently bound to the matrix.
A method of making amplified circular nucleic acids comprising attaching a plurality of circular nucleic acids together, amplifying the circular nucleic acids to produce amplicons, and covalently attaching the amplicons to the circular nucleic acids.
A detection reagent comprising at least one probe reagent and at least one nucleic acid label,
- wherein said at least one nucleic acid label comprises at least one pre-determined subsequence,
- wherein said at least one pre-determined subsequence forms an identifier of said at least one probe reagent, and
- wherein said at least one probe reagent and said at least one nucleic acid label are conjugated together.
Dependencies are inferred from the claim text.