Methods for analyzing cells
AssigneeCoral Genomics, Inc.
InventorsJacob BORRAJO, Atray DIXIT
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods for sample processing and analysis. A method of analyzing a plurality of cells may comprise providing a plurality of cells derived from cells of a plurality of subjects, which plurality of cells comprise nucleic acid molecules comprising barcode sequences identifying them as deriving from a subject of the plurality of subjects. Nucleic acid molecules derived from a plurality of nucleic acid molecules of the plurality of cells may be sequenced to provide a plurality of sequencing reads, and the resultant sequencing reads may be processed to associate a subset of the plurality of sequencing reads with a subject.
Claims21
-111. (canceled)
A method of analyzing a plurality of cells, comprising:
- (a) providing a plurality of cells derived from cells of a plurality of subjects, wherein said plurality of cells comprise a plurality of nucleic acid molecules, and wherein said plurality of nucleic acid molecules comprise a plurality of barcode sequences;
- (b) sequencing nucleic acid molecules derived from said plurality of nucleic acid molecules of said plurality of cells, thereby generating a plurality of sequencing reads corresponding to said plurality of nucleic acid molecules, wherein a portion of said plurality of sequencing reads comprise said plurality of barcode sequences;
- (c) processing said plurality of sequencing reads, which plurality of sequencing reads comprises said plurality barcode sequences;
- and
- (d) using a barcode sequence of said plurality of barcode sequences to associate a subset of said plurality of sequencing reads with a subject of said plurality of subjects,
- wherein, prior to (b), said plurality of cells is generated upon proliferating said cells of said plurality of subjects in a bulk growth environment.
Dependencies are inferred from the claim text.