Method for monitoring kinase activity in a sample
AssigneeUniversiteit Utrecht Holding B.V.
InventorsAdrianus Fredrik Maarten ALTELAAR, Thierry Thomas SCHMIDLIN
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Abstract
The present invention relates to a method for monitoring kinase activity or activation in a sample, the method comprises the steps of a) providing a sample comprising a kinase, b) incubating the sample with a protease to cleave the kinase provided in step a) into protease specific proteolytic peptides, c) applying phosphopeptide enrichment to the sample, d) analysing the sample obtained in step c) via liquid chromatography-mass spectrometry, and e) detecting phosphorylations of the kinase provided in step a), wherein the detection of step e) is performed only in case a proteolytic peptide associated with the activation region of the kinase is identified.
Claims17
A method for monitoring kinase activity or activation in a sample, the method comprises the steps of:
- a) providing a sample comprising a kinase;
- b) incubating the sample with a protease to cleave the kinase provided in step a) into protease specific proteolytic peptides;
- c) applying phosphopeptide enrichment to the sample;
- d) analysing the sample obtained in step c) via liquid chromatography-mass spectrometry;
- and
- e) detecting phosphorylations of the kinase provided in step a),
- wherein the detection of step e) is performed only in case a proteolytic peptide associated with the activation region of the kinase is identified.
Dependencies are inferred from the claim text.