Assays and methods for detecting udp-glucose
AssigneeThe General Hospital Corporation
InventorsSylvie Breton, Rachel Liberman, Leileata M. Russo
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Abstract
Disclosed herein are methods, assays, compositions and kits for the detection and measurement of UDP-glucose in biological samples. The methods, assays, compositions and kits are suitable for use with equipment that is readily available in a clinical laboratory, and permit rapid and reproducible detection and measurement of UDP-glucose at physiologically relevant levels in biological samples.
Claims21
(canceled)
A composition comprising uridine diphosphate glucose (UDP-glucose) dehydrogenase immobilized on a solid support.
A composition comprising uridine diphosphate glucose (UDP-glucose) dehydrogenase immobilized on a solid support selected from the group consisting of a lateral flow strip, lateral flow device, or a dipstick device, wherein the lateral flow strip, lateral flow device, or dipstick device comprises the following regions in order:
- (1) a sample application zone where the sample is applied and moving in the direction of capillary flow;
- (2) a test region comprising immobilized UDP-glucose dehydrogenase;
- (3) a region with a strong base or a region comprising immobilized NAD+ nucleosidase;
- and
- (4) a region comprising a detection reagent that provides an optically detectable readout product in the presence of NADH.
Dependencies are inferred from the claim text.