Back to results
US 2021/0262000 A1Published application
Glycosylation of proteins
AssigneeDanmarks Tekniske Universitet
InventorsBjørn Gunnar Voldborg, Stefan Kol, Anders Holmgaard Hansen, Helene Faustrup Kildegaard
Life of this patent
Estimating the term…
Abstract
The present invention relates to the finding of methods to shift the glycosylation profile of recombinant produced semm glycoproteins to the predominant bi-antennary form found in human plasma. This is accomplished by providing a mammalian cell line according to the invention with a series of gene disruptions and/or gene insertions that facilitate this shift.
Claims20
A recombinant mammalian cell line having a) one or more of the endogenous genes Mgat4A, Mgat4B, Mgat5, St3Gal3, St3Gal4, St3Gal6, SPPL3, and FUT8 inactivated and/or downregulated;
- and
- b) optionally a gene ST6Gal1 inserted.
Dependencies are inferred from the claim text.