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US 2021/0261998 A1Published application
Compositions and methods related to nucleic acid preparation
AssigneeNuclera Nucleics Ltd.
InventorsMichael C. Chen, Radu A. Lazar, Jiahao Huang, Gordon R. McInroy
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Abstract
The invention relates to a method of nucleic acid synthesis comprising the use of 3′-O-azidomethyl blocked nucleotide triphosphates which comprises the step of adding a capping group to any uncleaved 3′-O-azidomethyl groups and to the use of kits comprising said capping groups in a method of nucleic acid synthesis. The invention also relates to capped nucleotide triphosphates and 3′-O-azidomethyl capping groups.
Claims16
A method of treating an oligonucleotide, which comprises the steps of:
- (a) providing an oligonucleotide with a 3′-O-azidomethyl group an initiator sequence;
- and
- (b) treating the 3′-O-azidomethyl group via a 1,3-dipolar cycloaddition reaction to prevent subsequent cleavage of the 3′-O-azidomethyl group.
12-16. (canceled)
(canceled)
Dependencies are inferred from the claim text.