Methods and compositions for assessing crispr/cas-mediated disruption or excision and crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo

A method of testing the ability of a CRISPR/Cas nuclease to excise a genomic nucleic acid in vivo, comprising:

• (a) introducing into a non-human animal comprising a CRISPR reporter for assessing CRISPR/Cas-induced excision of a nucleic acid between first and second guide RNA target sequences:
• (i) a first guide RNA or a DNA encoding the first guide RNA, wherein the first guide RNA is designed to hybridize to the first guide RNA target sequence in the CRISPR reporter;
• (ii) a second guide RNA or a DNA encoding the second guide RNA, wherein the second guide RNA is designed to hybridize to the second guide RNA target sequence in the CRISPR reporter;
• and
• (iii) a Cas protein or a nucleic acid encoding the Cas protein;
• wherein the CRISPR reporter is integrated at a target genomic locus and comprises a first polyadenylation signal flanked by the first and second guide RNA target sequences followed by a reporter cassette comprising a coding sequence for a first reporter protein and a coding sequence for a second reporter protein in any order,
• wherein the first reporter protein and the second reporter protein are different;
• and
• (b) measuring the activity or expression of at least one of the first and second reporter proteins.