Resistance to soybean cyst nematode through gene editing

4-9. (canceled)

11-31. (canceled)

A method of generating variation in a C-terminal region of a soybean SNAP18 protein, comprising:

• introducing an editing system into a soybean plant cell, wherein the editing system is targeted to a region of an endogenous SNAP18 gene that encodes the C-terminal region of the SNAP18 protein;
• and
• contacting the region of the endogenous SNAP18 gene with the editing system, thereby introducing a mutation into the C-terminal region of the SNAP18 protein and generating variation in the C-terminal region of the soybean SNAP18 protein, optionally wherein generating variation in the C-terminal region of a soybean SNAP18 protein in a plant results in the plant having increased resistance to SCN or reduced severity of infection by SCN.

(canceled)

The method of claim 31, wherein contacting the region of the endogenous SNAP18 gene in the plant cell with the editing system produces a plant cell comprising in its genome an edited endogenous SNAP18 gene, the method further comprising (a) regenerating a plant from the plant cell;

• (b) selfing the plant to produce progeny plants (E1);
• (c) assaying the progeny plants of (b) for SCN resistance;
• and (d) selecting the progeny plants exhibiting increased SCN resistance or reduced severity of infection by SCN to produce selected progeny plants exhibiting increased SCN resistance or reduced severity of infection by SCN as compared to an unedited control plant.

38-44. (canceled)

(canceled)

(canceled)

(canceled)

A guide nucleic acid that binds to a target site in a SNAP18 gene, the target site comprising a portion of any one of the nucleotide sequences of SEQ ID NO:61-64.

59-69. (canceled)