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US 2021/0261931 A9Published application
Novel esterases and uses thereof
AssigneeCarbios
InventorsCHRISTOPHER TOPHAM, HÉLÈNE TEXIER, VINCENT TOURNIER, MARIE-LAURE DESROUSSEAUX, SOPHIE DUQUESNE, ISABELLE ANDRE, SOPHIE BARBE, ALAIN MARTY
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Abstract
The present invention relates to novel esterase, more particularly to esterase variants having improved thermostability compared to the esterase of SEQ ID No 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
Claims20
An esterase variant which (i) has at least 75% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, and (ii) has one or more amino acid modifications as compared to SEQ ID NO:
- 1 at position(s) selected from D203+S248, E173, L202, N204, A172+A209, V28, S29, R30, L31, S32, V33, S34, G35, F36, G37, G38, G39, A103, L82, G53, L104, L107, L119, A121, L124, I54, M56, L70, L74, A127, V150, L152, L168, V170, P196, V198, V200, V219, Y220, T221, S223, W224, M225, L239, T252, N253, H256, S1, Y4, Q5, R6, N9, S13, T16, S22, T25, Y26, S34, Y43, S48, T50, R72, S98, N105, R108, S113, N122, S145, K147, T160, N162, S181, Q189, N190, S193, T194, S212, N213, N231, T233, R236, Q237, N241, N243, N254, R255 and Q258 or at least one amino acid substitution selected from V177I, Y92G/P, F208I/W, T61M, wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.