Affinity carrier using mutant vhh antibody
AssigneeJsr Corporation
InventorsYoshihisa HAGIHARA, Yoko AKAZAWA, Yuji ITO, Tomonari MATSUDA, Norihiko KIYOSE, Nobuo MIYAZAKI, Tetsuo FUKUTA, Yusaku MIZUGUCHI
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Abstract
Provision of an affinity carrier using a mutant VHH antibody. An affinity carrier comprising: a solid phase carrier; and an immunoglobulin-binding protein bound to the solid phase carrier; wherein the immunoglobulin-binding protein comprises a mutant VHH antibody or a fragment of the mutant VHH antibody that recognizes an epitope in at least one region selected from the group consisting of amino acids 127 to 184 of SEQ ID NO: 22 and amino acids 13 to 210 of SEQ ID NO: 23.
Claims18
An affinity carrier comprising:
- a solid phase carrier;
- and
- an immunoglobulin-binding protein bound to the solid phase carrier,
- wherein the immunoglobulin-binding protein comprises a mutant VHH antibody or a fragment of the mutant VHH antibody that recognizes an epitope in at least one region selected from the group consisting of amino acids 127 to 184 of SEQ ID NO:
- 22 and amino acids 13 to 210 of SEQ ID NO:
- 23.
An affinity carrier comprising:
- a solid phase carrier;
- and
- an immunoglobulin-binding protein bound to the solid phase carrier,
- wherein the immunoglobulin-binding protein comprises a polypeptide consisting of an amino acid sequence having an identity of at least 85% to an amino acid sequence of SEQ ID NO:
- 1 in which at least at one position selected from the group consisting of amino acids 97, 100, and 102 of SEQ ID NO:
- 1 is histidine.
An affinity carrier comprising:
- a solid phase carrier;
- and
- an immunoglobulin-binding protein bound to the solid phase carrier,
- wherein the immunoglobulin-binding protein comprises a polypeptide consisting of an amino acid sequence having an identity of at least 85% to an amino acid sequence of SEQ ID NO:
- 1 in which at least at one position selected from the group consisting of amino acids 43, 53, 64, 75, and 86 of SEQ ID NO:
- 1 is arginine.
Dependencies are inferred from the claim text.