Automated device and method to purify biomaterials from a mixture by using magnetic particles and disposable product-contact materials
AssigneeSunil Mehta
InventorSunil Mehta
Life of this patent
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Abstract
This invention relates to a device and method of using the device for purification that separates material of interest from contaminating materials using non-porous magnetic particles and single-use or disposable materials that come in contact with the material of interest. The process encompasses multiple cycles in a single batch to reduce the cost of magnetic particles. This method can be executed in a fully automated manner by a controller that manages different inputs and outputs of system hardware.
Claims18
A device to separate material(s) of interest from a mixture containing plurality of materials, the device comprising:
- at least one disposable separation chamber with at least one inlet and one outlet;
- at least one disposable bag connected by tubing to inlet and outlet;
- at least one pump to move fluid in tubes;
- at least one valve to direct fluid in the system;
- at least one disposable connector and tubing to connect parts of the device
- at least one pH, conductivity, pressure, occlusion, bubble, or optical density sensor;
- a magnetic field generation unit that is controlled by a controller;
- particles that:
- (i) are mostly non-porous (ii) are attracted to magnetic field (iii) have outer surface with affinity for material of interest;
- a controller in communication with:
- (i) pumps to control their speed and direction, (ii) magnetic field unit to control the strength of magnetic field (iii) valves to control their open or closed state (iii) sensors to receive inputs from sensors (iv) a Human Machine Interface in which user inputs recipe parameters for a process and user can input to control pumps, valves, and magnetic field;
- wherein in operation, the controller changes the state of valves and creates a fluid path through which a pump transfers a fluid to the separation chamber;
- wherein in operation, the controller changes the state of valves and creates a fluid path through which a pump recirculates the fluid to provide mixing of the particles with the fluid,
- wherein in operation, the controller increases the strength of the magnetic field to immobilize particles in the separation chamber,
- wherein in operation, the controller changes the state of valves and creates a fluid path through which a pump transfers a fluid to a bag,
- wherein in operation, the controller lowers the strength of the magnetic field to suspend particles in the separation chamber.
A method of using the device to separate material(s) of interest from a mixture containing plurality of materials, the method comprising:
- mixing the starting material containing a mixture of materials with particles that are attracted to magnetic field and have specific affinity for a material of interest,
- capturing particles in the separation chamber by changing the strength of the magnetic field and discarding or collecting the flow-through material,
- washing particles with a buffer in the presence of magnetic field,
- washing particles by recirculating a buffer in the absence of magnetic field and then in the presence of magnetic field to retain particles in the separation chamber,
- discarding wash buffer, introducing elution buffer in the separation chamber, and mixing via pump recirculation in the absence of magnetic field followed by in the presence of magnetic field to elute the material specifically bound to the beads, and repeating this step to improve recoveries and to clean the beads.
Dependencies are inferred from the claim text.