Magnetic particle isolation device and methods of use

A particle isolation device, comprising:

• a) a fluidic channel structure comprising at least one input port and at least one output port interconnected by a fluidic channel, wherein the fluidic channel includes a substantially linear portion comprising a leading end that is in fluidic communication with the at least one input port and a terminal end that is in fluidic communication with the at least one output port;
• b) two magnetic components that are substantially vertically positioned on opposite sides of the substantially linear portion of the fluidic channel, wherein the two magnetic components are configured to create an asymmetric magnetic field within the substantially linear portion of the fluidic channel;
• and
• c) optionally, one or more pumps configured to drive fluid from the at least one input port through the fluidic channel and out the at least one output port.

A particle isolation device, comprising:

• a) a fluidic channel structure comprising at least one input port and at least two output ports interconnected by a series of fluidic channels, wherein the series of fluidic channels comprises a first fluidic channel comprising a first substantially linear portion, a second fluidic channel comprising a second substantially linear portion, and a third fluidic channel comprising a third substantially linear portion, wherein the fluidic channel structure comprises a first processing pathway that extends from the at least one input port to the first substantially linear portion and from the first substantially linear portion to the second substantially linear portion and from the second substantially linear portion to a first output port of the at least two output port, and a second processing pathway that extends from the at least one input port to the first substantially linear portion and from the first substantially linear portion to the third substantially linear portion and from the third substantially linear portion to a second output port of the at least two output ports;
• b) a first pair or magnetic components, a second pair of magnetic components, and a third pair of magnetic components that are substantially vertically positioned on opposite sides of the first substantially linear portion, the second substantially linear portion, and third substantially linear portion, respectively, wherein at least one of the first pair of magnetic components, second pair of magnetic components, or third pair of magnetic components is configured to create an asymmetric magnetic field within its adjacent substantially linear portion;
• and
• c) optionally, one or more pumps configured to drive fluid from the at least one input port through the series of fluidic channels and out the at least two output ports.

A method of isolating particles comprising:

• a) forming a processing solution comprising particles and a paramagnetic medium;
• b) passing the processing solution through a particle isolation device, wherein the device comprises a fluidic channel structure comprising a fluidic channel, and at least two magnetic components that are positioned on opposite sides of the fluidic channel, and wherein the two magnetic components create an asymmetric magnetic field within the fluidic channel, thereby isolating the particles as they pass through the asymmetric magnetic field, thereby generating isolated particles;
• and
• c) observing, analyzing, recording, and/or collecting the isolated particles.