Extracellular vesicles for the treatment and prevention of infections and other diseases
AssigneePhysis Biotechnologies, LLC
InventorsAngela Chukly, Emery Chukly
Life of this patent
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Abstract
In various embodiments, drug-loaded extracellular vesicles and methods of making the same are disclosed. In various embodiments, the EVs comprise natural exosomes or exomeres derived from cells that take part in the pathogenesis of a disease or a condition to which the drug-loaded EVs are targeted. In various embodiments, extracellular vesicles are surface modified, comprising bound biomolecules such as proteins or antibodies that can target ACE2 receptors. Surface modified EVs find use in the treatment and prevention of viral infections, such as COVID-19 caused by a SARS-CoV-2 infection in a human individual.
Claims20
A method of obtaining a subset of extracellular vesicles usable in the manufacture of drug-loaded extracellular vesicles configured to treat a disease or condition, the method comprising:
- choosing the disease or condition for treatment with drug-loaded extracellular vesicles;
- choosing a source of cells for naturally producing the extracellular vesicles by identifying the cells involved in a pathogenesis of the disease or the condition;
- culturing, harvesting and isolating the extracellular vesicles from the cell source;
- fractionating the characterized extracellular vesicles using a secondary assay method to obtain a subset of extracellular vesicles;
- and
- characterizing and identifying the subset of extracellular vesicles prior to the manufacture of drug-loaded extracellular vesicles configured for treatment of the disease or the condition.
A method of manufacturing drug-loaded extracellular vesicles configured for the treatment of a disease or a condition in a patient, the method comprising:
- culturing cells capable of naturally producing extracellular vesicles;
- isolating the naturally produced extracellular vesicles;
- purifying the extracellular vesicles;
- and
- incubating the purified extracellular vesicles in a buffer comprising the drug for a time sufficient to load the extracellular vesicles ex vivo with the drug to produce the drug-loaded extracellular vesicles.
A pharmaceutical composition configured to treat a disease or a condition, comprising:
- extracellular vesicles;
- and
- a drug encapsulated in the extracellular vesicles,
- wherein the extracellular vesicles are naturally produced from cells involved in a pathogenesis of the disease or the condition, and
- wherein the drug comprises at least one of an antibody, antibiotic, antiviral, antifungal, antitumor, a protein, a nucleic acid, an oligonucleotide, a chemotherapeutic, or a cannabinoid.
Dependencies are inferred from the claim text.