Method of treating diseases associated with myd88 pathways using crispr-gndm system

2:

• The method according to claim 1, wherein the MYD88 expression is suppressed without affecting the expression of an adjacent ACAA1 gene.

3:

• The method according to claim 1, wherein the disease is selected from the group consisting of an inflammatory disease, an autoimmune disease, an infectious disease and a cancer.

4:

• The method according to claim 1, wherein the disease is NASH.

5:

• The method according to claim 1, wherein the disease is a hematological malignancy.

6:

• A CRISPR-GNDM system for suppressing MYD88 expression comprising (a) a protein selected from the group consisting of dCas9 or dCpf1, and a fusion protein of dCas9 or dCpf1 and Kruppel associated box (KRAB) or other transcription repressor, and (b) a guide nucleotide (gN) targeting an expression regulatory region of MYD88 gene.

7:

• The CRISPR-GNDM system according to claim 6, wherein the protein of (a) is selected from the group consisting of dCas9 or dCpf1, and a fusion protein of dCas9 or dCpf1 and Kruppel associated box (KRAB).

8:

• The CRISPR-GNDM system according to claim 6, wherein the expression regulatory region is a region having the nucleotide sequence by set forth in SEQ ID NO:
• 65.

9:

• The CRISPR-GNDM system according to claim 6, wherein the expression regulatory region is a region having the nucleotide sequence set forth in positions 1,405-7,352 of SEQ ID NO:
• 65.

10:

• The CRISPR-GNDM system according to claim 6, wherein the gN comprises a nucleotide sequence set forth in any one of SEQ ID NOs:5 to 35.

11:

• The CRISPR-GNDM system according to claim 6, wherein the gN comprises a nucleotide sequence set forth in any one of SEQ ID NOs:9 to 35.

12:

• The method according to claim 1, wherein the CRISPR-GNDM system comprises (a) a protein selected from the group consisting of dCas9 or dCpf1, and a fusion protein of dCas9 or dCpf1 and Kruppel associated box (KRAB) or other transcription repressor, and (b) a guide nucleotide (gN) targeting an expression regulatory region of MYD88 gene.

13:

• The method according to claim 12, wherein the protein of (a) is selected from the group consisting of dCas9 or dCpf1, and a fusion protein of dCas9 or dCpf1 and Kruppel associated box (KRAB).

14:

• The method according to claim 12, wherein the expression regulatory region is a region having the nucleotide sequence set forth in SEQ ID NO:
• 65.

15:

• The method according to claim 12, wherein the expression regulatory region is a region having the nucleotide sequence set forth in positions 1,405-7,352 of SEQ ID NO:
• 65.

16:

• The method according to claim 12, wherein the gN comprises a nucleotide sequence set forth in any one of SEQ ID NOs:5 to 35.

17:

• The method according to claim 12, wherein the gN comprises a nucleotide sequence set forth in any one of SEQ ID NOs:9 to 35.