Solution for prolonging open time of fertilization hole of fish eggs and method for introducing exogenous gene through fertilization hole

Abstract

The present application provides a solution for prolonging open time of fertilization hole of fish eggs and a method for introducing exogenous gene through fertilization hole, belonging to the technical field of research and production of transgenic fish, wherein the solution takes water as a solvent and includes 6 g of sodium chloride, 0.15 g of potassium chloride, 0.1 g of calcium chloride, 0.1 g of sodium bicarbonate, 0.1 g of sodium dihydrogen phosphate and 1 g of glucose per liter. The solution provided by the present application can prolong the open time of the fertilization hole of the fish eggs, so that the exogenous genes can enter the fish eggs directly.

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