Enzyme-based electrochemical nicotine biosensor
AssigneeTrustees of Boston University
InventorsJames GALAGAN, Mark W. GRINSTAFF, Uros KUZMANOVIC, Mingfu CHEN, Margarita Alexandrovna TARARINA, Karen Nazaretian ALLEN
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Abstract
Described herein is an amperometric biosensor, e.g., chronoamperometric biosensor for the measurement of the concentration of nicotine. Also disclosed herein is a wearable nicotine biosensor device and a biosensor that detects nicotine in smoke. The biosensor disclosed herein comprises a nicotine-catalyzing enzyme, such as NicA2 or mutant NicA2 enzymes. Also described herein are systems comprising said amperometric biosensor, e.g., chronoamperometric biosensor and methods of using said chronoamperometric biosensor.
Claims28
A biosensor for the measurement of the concentration of nicotine comprising:
- a. an electrode comprising a surface;
- b. an electronically active mediator (Med) deposited on the surface of the electrode;
- and
- c. a plurality of nicotine-catalyzing enzymes deposited on the surface of the electrode, wherein the nicotine-catalyzing enzyme catalyzes nicotine to produce hydrogen peroxide (H2O2).
A wearable electrochemical nicotine biosensor device shown in FIGS. 29A and 29B.
Dependencies are inferred from the claim text.