Devices, methods, and systems for detecting and identifying bacteria based on fluorescence signature

A system for acquiring data regarding a target, comprising:

• a first excitation light source positioned to substantially uniformly illuminate a target surface with excitation light during imaging;
• and
• a portable housing configured to be held in a user's hand during fluorescent imaging, the housing containing:
• a plurality of selectable filters configured to permit optical signals responsive to illumination of the target surface and having a wavelength corresponding to bacterial, fungal, and/or other microorganism autofluorescence and bacterial, fungal, viral, and/or microbial fluorescence to pass through the filter, the plurality of selectable filters respectively corresponding to different discrete spectral bandwidths,
• an image sensor configured to detect the filtered optical signals, and
• a processor configured to receive the detected optical signals and to output data regarding a spatial distribution of an infection in the target surface based on the detected optical signals.

A method for acquiring data regarding a wound in tissue, comprising:

• illuminating a wound with excitation light comprising at least one wavelength or wavelength band causing at least one biomarker in the illuminated wound to fluoresce;
• filtering, with a first filter of a plurality of selectable filters included in a spectral filtering mechanism, optical signals emitted in response to illumination of the wound with the excitation light, the spectral filtering mechanism being configured to enable optical signals having a wavelength corresponding to bacterial, fungal, and/or other microorganism autofluorescence and/or bacterial, fungal, viral, and/or microbial fluorescence to pass through the spectral filtering mechanism, wherein the plurality of selectable filters respectively correspond to different discrete spectral bandwidths;
• collecting, through the first filter, bacterial, fungal, and/or other microorganism autofluorescence data and/or bacterial, fungal, viral, and/or microbial fluorescence data regarding the wound with an optical sensor configured to detect the spectrally filtered signals;
• receiving the collected bacterial, fungal, and/or other microorganism autofluorescence data and/or bacterial, fungal, viral, and/or microbial fluorescence data at a processor and identifying a fluorescent signature of bacteria, fungus, virus, and/or other microbe in and/or around the wound based at least in part on the collected bacterial, fungal, and/or other microorganism autofluorescence data and/or bacterial, fungal, viral, and/or microbial fluorescence data and outputting data regarding the bacterial, fungal, viral, and/or microbial fluorescent signature.