Generation of haploid plants

A plant having biological activity of a haploid inducer and comprising a nucleotide sequence encoding a centromere histone H3 (CENH3) protein comprising a CATD domain, wherein the nucleotide sequence comprises a mutation encoding a polypeptide having one or more amino acid substitutions at positions 2, 6, and 12 of a loop1 consensus sequence, wherein said alteration confers the biological activity of a haploid inducer, wherein the plant is homozygous with respect to the mutation and is not able to express a native CENH3 protein, and

• wherein the loop1 consensus sequence comprises:
• T, S, or A at position 1:
• H, Q, N, A, Y, F, G, D or E at position 2;
• M, Q, I, F, Y, A, E, N, R, L, H or G at position 3;
• L, F, V, I or Y at position 4;
• A, T, S, C or M at position 5;
• P, N, D, R, A, T, F, R, H, S or K at position 6;
• Q, Y, D, K, R, E, G, S, P, H, N or A at position 8;
• I, V or P at position 9;
• N, G, T, E or S at position 10;
• R or P at position 11;
• W or Y at position 12;
• and T, Q or S at position 13.