Polymer coatings
AssigneeIllumina, Inc.
InventorsWayne N. George, Andrew A. Brown, Daniel Bratton, Hongji Ren, Ryan Christopher Smith
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.
Claims21
-49. (canceled)
A substrate comprising a first plurality of oligonucleotides and a second plurality of oligonucleotides immobilized on a surface of the substrate through a polymer that is covalently attached to the surface of the substrate, wherein the polymer comprising a recurring unit of Formula (Ia) and a recurring unit of Formula (II):
- wherein:
- R1 is H, alkyl, alkoxy, alkenyl, alkynyl, or optionally substituted variants thereof;
- RA is azido;
- each of the —(CH2)o— units is optionally substituted;
- o is an integer between 1 and 50;
- each R4, R4′, R5 and R5′ is independently selected from the group consisting of H, R6, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O)NR7R8, and —NR7R8;
- each R6 is independently H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, or heterocyclyl, or an optionally substituted variant thereof;
- and
- each R7 and R8 is independently H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle.
Dependencies are inferred from the claim text.