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US 2021/0246479 A1Published application
Method for Secretory Production of Protein
AssigneeAjinomoto Co., Inc.
InventorYoshihiko Matsuda
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Abstract
A novel technique for reducing the mis-cleavage of the TorA signal peptide, and thereby a method for efficient secretory production of a heterologous protein by a coryneform bacterium using a TorA signal peptide is provided. A coryneform bacterium having an ability of secretory production of a heterologous protein using a TorA signal peptide and has been modified so that the activity of a LepB protein is increased is cultured to produce the heterologous protein by secretory production.
Claims17
A method for producing a heterologous protein comprising:
- culturing a coryneform bacterium having a genetic construct for secretory expression of the heterologous protein;
- and
- collecting the heterologous protein produced by secretory production,
- wherein the coryneform bacterium has been modified so that the activity of a LepB protein is increased,
- wherein the genetic construct comprises, in the direction from 5′ to 3′, a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence encoding a TorA signal peptide, and a nucleic acid sequence encoding the heterologous protein, and
- wherein the heterologous protein is expressed as a fusion protein with the TorA signal peptide.
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