Biosynthesis of preparing nicotinamide mononucleotide and derivatives thereof

A method of making nicotinamide mononucleotide (NMN), NMN derivatives, or a mixture thereof, comprising generating a multi-enzyme reaction by mixing in an aqueous solution:

• i. hypoxanthine/guanine phosphoribosyltransferase and nicotinamide phosphoribosyltransferase,
• ii, nicotinamide,
• iii, inosine monophosphate, guanine monophosphate, or a mixture thereof,
• iv. 5-phospho-alpha-D-ribose-1-diphosphate (PRPP),
• v. inorganic diphosphate anions (PPi), and
• vi. Mg2+,
• wherein:
• the hypoxanthine/guanine phosphoribosyltransferase synthesizes PRPP and a purine base from nucleotides and PPi, the nucleotides comprising inosine monophosphate or guanine monophosphate and the purine base comprising inosinate or guanine;
• the nicotinamide phosphoribosyltransferase synthesizes nicotinamide mononucleotide from PRPP and nicotinamide, releasing PPi to provide a substrate for the hypoxanthine/guanine phosphoribosyltransferase;
• and
• wherein byproducts of the multi-enzyme reaction are removed in situ and converted to non-inhibitory chemicals with supplementary enzymes driving the multi-enzyme reaction toward the NMN synthesis.