Bio-imaging probes and methods for non-invasive detection of human uterine sarcomas
OwnerAyman Al-Hendy, Natalia Garciainventors
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Abstract
This invention provides compositions of a viral vector system containing a survivin promotor configured for expressing a reporter gene, wherein the reporter gene is differentially expressed in benign and malignant uterine masses and malignant uterine sarcomas can be bioimaged by preferential labeling with a radiotracer.
Claims21
An adenovirus Survivin-Sodium iodide symporter (Ad-SUR-NIS) vector composition comprising:
- a Survivin promoter operatively linked to a sodium iodine symportor protein (NIS) protein-encoding sequence that is capable of infecting leiomyosarcoma (LMS) cells in vivo.
5-7. (canceled)
A method of detecting uterine sarcoma in a woman, the method comprising:
- administering to the subject a composition comprising an adenovirus Survivin-Sodium iodide symporter Ad-SUR-NIS vector and a radiotracer;
- exposing a uterus of the woman where the presence of a sarcoma is suspected to PET-CT imaging;
- and
- capturing energy emitted from the target area of the subject in the imaging data, wherein the presence of the energy emitted correlates to symporter expression uptake of a radiotracer indirectly indicating the presence of a sarcoma in the target area of the subject.
(canceled)
A pre-surgical diagnostic system for detecting a leiomyosarcoma (LMS) based on survivin expression in uterine sarcoma of a subject comprising:
- an adenovirus Survivin-Sodium iodide symporter (Ad-SUR-NIS) composition configured for delivery to the LMS in a weight dosage concentration selected from a uterine mass weight dose range;
- and
- a radiotracer configured to emit energy based on gene expression of the Ad-SUR-NIS symporter and uptake of the radiotracer thereby;
- wherein
- an imaging device configured to capture energy signals emitted from the radiotracer as an indirect confirmation of the presence of a leiomyosarcoma (LMS).
14-21. (canceled)
(canceled)
A method of detecting sarcoma in a subject, the method comprising the steps of:
- administering a composition comprising a viral vector containing a survivin promotor configured to express a sodium iodine symportor protein (NIS) protein in the subject;
- delaying administration of a radiotracer for a delay time until the viral vector reaches a target area in the subject to express the NIS protein in the mass;
- administering the radiotracer after the delay time, where the radiotracer can be selected from Table 2;
- exposing the target area to PET-CT imaging to generate imaging data;
- and
- capturing energy emitted from the target area of the subject in the imaging data, wherein the presence of the energy emitted correlates to uptake of a radiotracer due to NIS expression indicating the presence of a cancerous mass in the target area of the subject.
30-36. (canceled)
Dependencies are inferred from the claim text.