Method and means for the rapid detection of hdv infections
AssigneeUniversität Heidelberg
InventorsFLORIAN LEMPP, STEPHAN URBAN
Life of this patent
Estimating the term…
Abstract
The present invention relates to a polypeptide and a nucleic acid encoding the polypeptide for use in a method of detecting the presence of hepatitis D vims (HDV) and/or of diagnosing an HDV infection and/or of monitoring the treatment of an HDV infection. The present invention further relates to an in vitro method, an immunographic test device as well as a kit. In particular, the present invention relates to a point of care diagnostic for HDV infections.
Claims21
A polypeptide comprising an amino acid sequence selected from
- an amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2,
- an amino acid sequence comprising or consisting of amino acid residues 1 to 195 of SEQ ID NOs:
- 1 or 2,
- an amino acid sequence comprising amino acid residues 60 to 214 of SEQ ID NOs:
- 1 or 2, and
- an amino acid sequence having at least 99% sequence identity to SEQ ID NO:
- 1.
3-6. (canceled)
An immunographic device for in vitro detecting the presence of Hepatitis D virus (HDV) in a sample of a subject, diagnosing an HDV infection and/or monitoring the treatment of an HDV infection, said device comprising a solid carrier coated with an anti-HDV IgG antibody binding agent,
- wherein the anti-HDV IgG antibody binding agent is the polypeptide of claim 1.
7 An in vitro method for detecting the presence of hepatitis D virus (HDV) and/or for diagnosing an HDV infection and/or for monitoring the treatment of an HDV infection in a sample of a subject, said method comprising:
- providing the polypeptide of claim 1;
- and
- detecting for IgG antibodies against the Hepatitis Delta Antigen (HDAg) in said sampler.
The method of claim 7, wherein the sample is serum, plasma, whole blood or saliva,
- and/or wherein HDV and HDV infections of all genotypes can be detected,
- and/or wherein one or more further infections are detected, such as HBV infection.
The immunographic device of claim 9, wherein the immunographic device comprises a porous membrane operably connected to
- (a) a sample portion/pad,
- (b) a conjugate portion/pad,
- (c) a test portion/line comprising said anti-HDV IgG antibody binding agent,
- (d) a control portion/line;
- and
- (e) an absorbent portion/pad.
The immunographic device of claim 9, wherein the immunographic device is a lateral flow assay (LFA) device.
The immunographic device of claim 9, wherein the sample is serum, plasma, whole blood or saliva,
- and/or wherein HDV and HDV infections of all genotypes can be detected,
- and/or wherein one or more further infections are detected.
A kit for in vitro detecting the presence of Hepatitis D virus (HDV) in a sample of a subject, diagnosing an HDV infection and/or monitoring the treatment of an HDV infection, wherein the kit comprises:
- a) an immunographic device according to claim 9;
- and
- b) instructions for using the immunographic device to detect the presence of said anti-HDV IgG antibodies in the sample
- and/or HDV and HDV infections of all genotypes can be detected,
- and/or further infections are detected, such as HBV infection.
The method of claim 7, which is a point of care method.
Dependencies are inferred from the claim text.