# US 2021/0239698 A1 · Identification of ligands and their use

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2021/0239698 A1
- Document ID: US20210239698A1
- Status: Published application
- Kind: A1
- Application number: 17/231,689
- Filed: 2021-04-15
- Published: 2021-08-05
- Earliest claimed: 2010-08-17
- Assignees: Oxford University Innovation Limited
- Applicants: Oxford University Innovation Limited
- Inventors: Graham Ogg; Li-Chieh Huang; Aamir Aslam
- Web page: https://patentgrep.com/patent/US20210239698A1

## CPC

- G01N 33/56977: {HLA or MHC typing}
- C12N 15/1055: {Protein x Protein interaction, e.g. two hybrid selection}
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/56972: {White blood cells}
- C07K 17/00: Carrier-bound or immobilised peptides (carrier-bound or immobilised enzymes C12N11/00); Preparation thereof
- G01N 2333/7051: T-cell receptor (TcR)-CD3 complex
- G01N 2333/70539: MHC-molecules, e.g. HLA-molecules
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The invention provides carriers and kits for use in the identification of ligands. Carriers of the invention are provided to which is attached a DNA encoding a peptide and a β2 microglobulin. Carriers of the invention are capable of identifying an epitope recognized by a T-cell receptor and/or an NK-cell receptor and/or an NKT-cell receptor. The invention also provides kits for screening for a T-cell epitope and/or an NK-cell epitope and/or an NKT-cell epitope, wherein the kits comprise a carrier of the invention.

## Claims

### Claim 1

A carrier to which is attached a peptide, DNA encoding the peptide, and β2 microglobulin.

### Claim 2 (depends on 1)

The carrier of claim 1 to which an MHC or MHC-like molecule is attached.

### Claim 3 (depends on 1)

The carrier of claim 1 wherein the β2 microglobulin is attached to the carrier as part of a larger construct which includes the peptide.

### Claim 4 (depends on 1)

The carrier of claim 1 wherein the peptide and β2 microglobulin are provided connected by a flexible linker.

### Claim 5 (depends on 1)

The carrier of claim 1 wherein the peptide is between about 4 and about 20 amino acids.

### Claim 6 (depends on 1)

The carrier of claim 1 wherein the carrier is a solid support.

### Claim 7 (depends on 1)

The carrier of claim 1 wherein the carrier is a bead.

### Claim 8 (depends on 1)

The carrier of claim 1 wherein the carrier is multivalent.

### Claim 9

A carrier to which is attached a peptide, DNA encoding the peptide, and β2 microglobulin and/or an MHC or MHC-like molecule.

### Claim 10 (depends on 1)

The use of a carrier according to claim 1 to identify an epitope recognised by a T-cell receptor and/or an NK-cell receptor and/or an NKT-cell receptor.

### Claim 11

A kit for screening for a T-cell epitope and/or an NK-cell epitope and/or an NKT-cell epitope, wherein the kit comprises a carrier to which is attached a peptide, DNA encoding the peptide and β2 microglobulin.

### Claim 12 (depends on 11)

The kit of claim 11 further including instructions to fold the peptide and β2 microglobulin.