Platform for Native Liquid Chromatography-Mass Spectrometry
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShunhai Wang, Yuetian Yan
Life of this patent
Estimating the term…
Abstract
Disclosed are native liquid chromatography-mass spectrometry systems and methods of use. A native liquid chromatography-mass spectrometry system can include a liquid chromatography system capable of separating a sample; and an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter and a system for modifying a desolvation gas and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.
Claims32
A native liquid chromatography-mass spectrometry system, comprising:
- a liquid chromatography system capable of separating a sample;
- and
- an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.
3-4. (canceled)
6-7. (canceled)
11-12. (canceled)
(canceled)
(canceled)
A method of characterizing a protein in a sample, comprising:
- supplying the sample to a liquid chromatography system capable of sample separation and fragmentation, the liquid chromatography system comprising a size exclusion chromatography system (SEC) column or an ion exchange chromatography (IEX) column;
- analyzing the fragmented sample by use of an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions to identify the components of the protein to characterize the protein;
- and
- wherein the method does not require deglycosylation of the protein in the sample prior to supplying the sample to the liquid chromatography system.
23-25. (canceled)
27-28. (canceled)
30-31. (canceled)
(canceled)
35-36. (canceled)
38-39. (canceled)
(canceled)
Dependencies are inferred from the claim text.