# US 2021/0239604 A1 · Optical measuring unit and optical measuring method for obtaining measurement signals of fluid media > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2021/0239604 A1 - Document ID: US20210239604A1 - Status: Published application - Kind: A1 - Application number: 17/049,833 - Filed: 2019-04-12 - Published: 2021-08-05 - Earliest claimed: 2018-04-23 - Assignees: Meon Medical Solutions GmbH & Co KG - Applicants: Meon Medical Solutions Gmbh & Co KG - Inventors: Herfried Huemer; Arnold Bartel; Stefan Gulo; Patrick Kraus-Füreder; Robert Scholz-Mareich; Wolfgang Sprengers - Web page: https://patentgrep.com/patent/US20210239604A1 ## CPC - G01N 21/31: Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} - B01L 3/5085: {for multiple samples, e.g. microtitration plates} - G01N 21/0303: {Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment (G01N21/031 - G01N21/15 take precedence)} - G01N 21/253: {for batch operation, i.e. multisample apparatus (analytical automats G01N35/00)} - G01N 21/51: inside a container, e.g. in an ampoule (G01N21/53 takes precedence) - B01L 2300/0654: Lenses; Optical fibres - B01L 2300/0829: Multi-well plates; Microtitration plates - B01L 2300/0832: cylindrical, tube shaped - B01L 2300/168: Specific optical properties, e.g. reflective coatings - G01N 2021/0389: {Windows} - G01N 2201/062: LED's - G01N 2201/0627: Use of several LED's for spectral resolution - G01N 2201/0633: Directed, collimated illumination - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract Aspects of the present disclosure are directed to, for example, an optical measurement unit for obtaining measurement signals from liquid media which are present in cuvettes lined up next to one another. In one embodiment, the optical measurement unit includes a light-supplying unit for emitting an inlet radiation into the cuvettes, and a detection unit for detecting a measurement radiation exiting from the cuvettes and for converting the measurement radiation into an electrical measurement signal. In such an embodiment, the light-supplying unit has a plurality of LED light sources which emit in a spectrally different manner in the UV/VIS/NIR wavelength range, and wherein the detection unit includes at least one photodiode fixedly assigned to each cuvette of a cuvette array. ## Claims ### Claim 1 An optical measurement unit for obtaining measurement signals from liquid media which are present in cuvettes lined up next to one another, each cuvette having a lateral inlet window and at least one lateral outlet window, the optical measurement unit comprising: a light-supplying unit configured and arranged for emitting spectrally different inlet radiations in temporal succession into the inlet window of each cuvette, the light-supplying unit having a plurality of LED light sources which emit in a spectrally different manner in the UV/VIS/NIR wavelength range, and a detection unit configured and arranged for detecting a measurement radiation exiting from the at least one outlet window of each cuvette and for converting the measurement radiation into an electrical measurement signal, wherein the cuvettes lined up next to one another form a stationary cuvette array, and the detection unit includes a plurality of photodiodes, at least one of the plurality of photodiodes being fixedly assigned to each outlet window of each cuvette of the cuvette array. ### Claim 2 (depends on 1) The optical measurement unit according to claim 1, wherein the plurality of photodiodes are arranged on a common circuit board at a distance from the cuvettes of the stationary cuvette array, wherein a preamplifier is arranged within each of the plurality of photodiodes or directly at the signal output of each photodiode. ### Claim 3 (depends on 1) The optical measurement unit according to claim 1, wherein the light-supplying unit further includes at least one stationary light distributor device configured and arranged to distribute the light from the plurality of LED light sources among the cuvettes, wherein the at least one stationary light distributor device has a cavity, the inner surfaces of which are at least partially mirrored and/or diffusely reflective, and wherein the at least one stationary light distributor device includes, for each of the plurality of LED light sources, an inlet opening configured and arranged for feeding the light into the cavity, and wherein the at least one stationary light distributor device has, for each cuvette of the cuvette array, an outlet opening for feeding the light into the cuvette. ### Claim 4 (depends on 3) The optical measurement unit according to claim 3, wherein the inner surface of the at least one stationary light distributor device that is located opposite the outlet openings to the cuvettes is designed to be diffusely reflective. ### Claim 5 (depends on 3) The optical measurement unit according to claim 3, wherein the inner surface of the at least one stationary light distributor device is located opposite the inlet openings of the plurality of LED light sources, and the inner surface is corrugated and reflective. ### Claim 6 (depends on 3) The optical measurement unit according to claim 3, wherein, in order to improve the spectral characteristic of the optical measurement unit, at least some of the plurality of LED light sources include optical filters. ### Claim 7 (depends on 6) The optical measurement unit according to claim 6, wherein the optical filter is at least one narrowband interference filter, and further includes at least one optical element configured and arranged for collimating the light and is arranged in the light path on the input side of the at least one narrowband interference filter. ### Claim 8 (depends on 6) The optical measurement unit according to claim 6, wherein at least one of the plurality of LED light sources are configured and arranged to collimate the emitted light via an LED arranged in a TIR lens. ### Claim 9 (depends on 6) The optical measurement unit according to claim 6, further including a tubular body configured and arranged for eliminating non-parallel beam components, the tubular body is arranged in the light path on the input side of the optical filter, wherein the tubular body includes through-openings parallel to the longitudinal axis thereof, walls of said through-openings including a light-absorbing material or are coated with a light-absorbing material. ### Claim 10 (depends on 7) The optical measurement unit according to claim 7, further including a converging lens arranged on the input side of the interference filter, the converging lens configured and arranged to align in parallel with the light emitted by at least one of the plurality of LED light sources. ### Claim 11 (depends on 10) The optical measurement unit according to claim 10, further including a diverging lens arranged on the output side of the interference filter, the diverging lens configured and arranged to fan out the radiation entering the at least one light distributor device. ### Claim 12 (depends on 3) The optical measurement unit according to claim 3, further including channel-like feedthroughs arranged in a wall of a cuvette receptacle on the entry side of the inlet window and on the exit side of the outlet window, said channel-like feedthroughs having fittings or modifications which are configured and arranged to eliminate undesired radiation components of the inlet radiation exiting from the at least one light distributor device and of the measurement radiation exiting from the cuvette. ### Claim 13 (depends on 12) The optical measurement unit according to claim 12, wherein the fittings or modifications of the channel-like feedthroughs in the cuvette receptacle of each cuvette are designed (1) as a channel having a smooth surface with a diameter smaller than the length, or (2) as a feedthrough having a cavity or a clearance, or (3) as a feedthrough having a fluted or toothed structure. ### Claim 14 (depends on 12) The optical measurement unit according to claim 12, wherein the walls of the channel-like feedthroughs in the cuvette receptacle consist of a light-absorbing material or are coated with a light-absorbing material. ### Claim 15 (depends on 3) The optical measurement unit according to claim 3, further including reference detectors arranged on the at least one light distributor device on the outlet side of through-openings or pinhole diaphragms arranged in a wall of the at least one light distributor device. ### Claim 16 (depends on 3) The optical measurement unit according to claim 3, wherein the stationary cuvette array is segmented, and the optical measurement unit further includes a separate light-supplying unit fixedly assigned to each segment of the stationary cuvette array. ### Claim 17 (depends on 1) The optical measurement unit according to claim 1, wherein the light-supplying unit has at least one unidimensional, rod-shaped light source array including a plurality of LED light sources, the light source array is oriented along the stationary cuvette array and is movable along the stationary cuvette array such that each of the plurality of LED light sources of the light source array are configured and arranged to be assigned to each cuvette of the stationary cuvette array. ### Claim 18 (depends on 17) The optical measurement unit according to claim 17, wherein one of the plurality of LED light sources is arranged together with a beam splitter and a reference detector in a common housing. ### Claim 19 (depends on 17) The optical measurement unit according to claim 17, wherein at least some of the plurality of LED light sources of the rod-shaped light source array have optical elements configured and arranged for collimation purposes, a narrowband filter and a condenser configured and arranged for focusing the light into the cuvette, wherein the optical elements, the narrowband filter and the condenser are configured and arranged to improve the spectral characteristic and to feed the light into the cuvettes. ### Claim 20 (depends on 1) The optical measurement unit according to claim 1, wherein the plurality of LED light sources are arranged as a 2D LED array fixedly assigned to each cuvette of the stationary cuvette array. ### Claim 21 (depends on 1) The optical measurement unit according to claim 1, wherein the plurality of LED light sources are arranged as a single 2D LED array, wherein the light-supplying unit is configured and arranged to be movable along the stationary cuvette array or a segment of the cuvette array such that the single 2D LED array can be assigned to each cuvette of the cuvette array or of a segment of the cuvette array. ### Claim 22 (depends on 20) The optical measurement unit according to claim 20, further including a 2D lens array configured and arranged for collimating the light from the individual LEDs, a 2D filter array configured and arranged for narrowband filtering of the light, and at least one condenser, the 2D lens array, 2D filter array and the at least one condenser configured and arranged to improve the spectral characteristic and to feed and focus the light from the individual LEDs of the 2D LED array into the cuvettes of the cuvette array. ### Claim 23 (depends on 22) The optical measurement unit according to claim 22, wherein the 2D LED array consists of LED emitters bonded to a single substrate, and wherein the 2D lens array is a 2D microlens array and the 2D filter array is a 2D microinterference filter array. ### Claim 24 (depends on 22) The optical measurement unit according to claim 22, wherein in each case one LED light source includes a 2D LED array, a 2D lens array, a 2D filter array and a converging lens, and each of the LED light sources is arranged together with a beam splitter and a reference detector in a common housing. ### Claim 25 (depends on 17) The optical measurement unit according to claim 17, wherein the detection unit has, coming from each cuvette of the stationary cuvette array, a receptacle with optical elements configured and arranged for focusing the measurement radiation onto the photodiode. ### Claim 26 An optical measurement method for obtaining measurement signals from liquid media, comprising the following steps: holding the liquid media in cuvettes which are lined up next to one another and which form a stationary cuvette array, supplying an inlet radiation, which radiates into the cuvettes, by means of at least one stationary light distributor device which optically contacts at least one segment of the cuvette array, wherein light is irradiated into the at least one stationary light distributor device in temporal succession by a plurality of LED light sources which emit in a spectrally different manner in the UV/VIS/NIR wavelength range, and detecting the radiation exiting from the cuvettes by means of at least one photodiode, fixedly assigned to each cuvette, of a stationary detection unit. ### Claim 27 (depends on 26) The method according to claim 26, wherein the spectral channels of the individual LED light sources are activated and deactivated in a predefined order, wherein in each case the photodiode arranged in a first cuvette position is detected, and in that, after running through all the spectral channels in the first cuvette position, a changeover to the next cuvette position takes place. ### Claim 28 (depends on 27) The method according to claim 27, wherein after each deactivation of one of the LED light sources, a dark measurement is carried out on the respective photodiode. ### Claim 29 (depends on 26) The method according to claim 26, wherein the spectral channel of the first LED light sources is activated, with the photodiodes arranged in the cuvette positions being detected in a predefined order, and in that, after running through all the cuvette positions, the next spectral channel of the next LED light sources is activated. ### Claim 30 (depends on 29) The method according to claim 29, wherein prior to any changeover to the next spectral channel, a dark measurement is carried out on the respective photodiode.