US 2021/0238640 A1 · PROCESS OF PRODUCING MONOTERPENES

Abstract

The present invention relates to a process of producing a monoterpene and/or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and/or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and/or derivatives thereof and a recombinant microor-ganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and/or derivatives thereof by ex-pression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.

Claims12

Dependent claims

A process of producing linalool in a host microorganism comprising the following steps:

(a) providing a host microorganism genetically modified to express a bacterial monoterpene synthase (mTS), an acetoacetyl-CoA thiolase (AtoB), a hydroxymethylglutaryl-CoA synthase (HMGS), a hydroxymethylglutaryl-CoA reductase (HMGR), a mevalonate kinase (MK), a phosphomevalonate kinase (PMK), a phosphomevalonate decarboxylase (PMD) and an isopentenyldiphosphate isomerase (idi);
(b) converting acetyl CoA into isopentenyl diphosphate (IPP);
(c) converting IPP and dimethylallyl diphosphate (DMAPP) into geranyl pyrophosphate (GPP);
and
(d) contacting GPP with said bacterial mTS to produce linalool,
wherein the bacterial mTS comprises linalool synthase, and
wherein the process results in a linalool yield of at least 100 mg Lorg−1.

4 more elements

Dependencies are inferred from the claim text.

Process of producing monoterpenes

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Process of producing monoterpenes

Life of this patent

Abstract

Claims12