Real-time and label free analyzer for in-vitro and in-vivo detecting the suspicious regions to cancer

7- The method of claim 1, wherein detecting the cancerous tumor at the target location comprises:

• generating the set of reference values, comprising:
• recording a set of CV responses from a plurality of samples using the fabricated probe, the plurality of samples comprising a plurality of healthy samples and a plurality of cancerous samples;
• and
• measuring a respective set of oxidation current peaks of the recorded set of CV responses;
• comparing the measured oxidation current peak with the generated set of reference values;
• and
• detecting the cancerous tumor at the target location responsive to the measured oxidation current peak being in a range of the generated set of reference values associated with the plurality of cancerous samples.

8- The method of claim 1, wherein fabricating the probe comprises:

• forming the working electrode and the counter electrode, comprising:
• coating two layers of silver (Ag) paste on respective tips of each of the first needle and the second needle, comprising:
• coating a first layer of Ag paste on the first tip of the first needle;
• and
• coating a second layer of Ag paste on the second tip of the second needle;
• electrically isolating rest part of each of the first needle and the second needle;
• and
• depositing CNTs on the two layers of Ag paste by electrostatically depositing CNTs powder on the two layers of Ag paste, comprising:
• depositing the first layer of CNTs on the first layer of Ag paste;
• and
• depositing the second layer of CNTs on the second layer of Ag paste;
• placing the working electrode and the counter electrode inside respective hollow needles of the two reference electrodes;
• and
• attaching the two reference electrodes, the working electrode and the counter electrode to three respective pins of an electrical connector.

10- The method of claim 8, wherein depositing CNTs on the two layers of Ag paste comprises:

• connecting CNTs powder to a positive pole of an electrostatic potential generator;
• connecting the first needle with Ag paste coated on the first tip and the second needle with Ag paste coated on the second tip to a negative pole of the electrostatic potential generator;
• and
• electrostatically grasping CNTs powder on the two layers of Ag paste by moving the first needle with Ag paste coated on the first tip and the second needle with Ag paste coated on the second tip alongside the positive pole of the electrostatic potential generator.

12- A probe for non-invasive detecting and tracing cancer, comprising:

• a sensing part, comprising:
• two reference electrodes comprising a first hollow needle and a second hollow needle;
• a working electrode comprising a first needle comprising a first tip coated with a first layer of carbon nanotubes (CNTs), the working electrode located inside the first hollow needle;
• and
• a counter electrode comprising a second needle comprising a second tip coated with a second layer of CNTs, the counter electrode located inside the second hollow needle;
• and
• a holding member, the sensing part attached to a first end of the holding member, the holding member comprising a button at a second end of the holding member, the button configured to:
• push the working electrode and the counter electrode outside the two reference electrodes, and
• pull the working electrode and the counter electrode inside the two reference electrodes.